Mouse Integrin alpha 2/CD49b Antibody

Name: Mouse Integrin alpha 2/CD49b Antibody
Brand: R&D Systems
SKU: MAB1740
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # MAB1740

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2B Clone # 235033

View all Integrin alpha 2/CD49b Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b
Tyr27-Thr1129
Accession # Q62469

Specificity

Detects mouse Integrin alpha 2/CD49b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 3, alpha 4, alpha 5, alpha E, or alpha V is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse Integrin alpha 2/CD49b Antibody

Detection of Integrin alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with (A) Rat Anti-Mouse Integrin a2/CD49b Monoclonal Antibody (Catalog # MAB1740) or (B) Rat IgG2B isotype control antibody (MAB006), followed by APC-conjugated Anti-Rat IgG Secondary Antibody (F0113) and Rat anti-Mouse NKp46 PE-conjugated Monoclonal Antibody (FAB22252P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Mouse Integrin alpha 2/CD49b Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse splenocytes

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin alpha 2/CD49b

The Integrin alpha 2 subunit, also known as CD49b and VLA-2 alpha subunit, forms a heterodimer with the Integrin alpha 1 (CD29) or alpha 4 (CD104) subunits and binds Laminin.