Mouse Integrin  alpha E/CD103 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1990G
Mouse Integrin  alpha E/CD103 Alexa Fluor® 488-conjugated Antibody in Data
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Mouse Integrin  alpha E/CD103 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha E/CD103 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 2, rmIntegrin alpha 3, rmIntegrin alpha 4, recombinant human (rh) Integrin alpha M, and rhIntegrin alpha V is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha E/CD103
Phe20-Arg1113 (Ser453Gly)
Accession # ABD49099
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Integrin  alpha E/CD103 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of Integrin alpha E/CD103 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse Integrin aE/CD103 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1990G) and Rat Anti-Mouse CD8a APC-conjugated Monoclonal Antibody (Catalog # FAB116A). Quadrant markers were set based on control antibody staining (Catalog # IC108G). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha E/CD103

The Integrin  alpha E subunit, also known as CD103, forms a non-covalent heterodimer with Integrin  beta 7. Integrin  alpha E/ beta 7 is a receptor for E-Cadherin. Integrin  alpha E is expressed in intra-epithelial lymphocytes and mediates adhesion of intra-epithelial lymphocytes to epithelial cell monolayers.

Entrez Gene IDs
3682 (Human); 16407 (Mouse); 83577 (Rat)
Alternate Names
antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103 antigen; CD103; HML-1 antigen; HUMINAE; Integrin alpha E; integrin alpha-E; Integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide); ITGAE; MGC141996; Mucosal lymphocyte 1 antigen

Product Datasheets

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