Mouse Integrin  alpha E/CD103 Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Integrin alpha E/CD103 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 2, rmIntegrin alpha 3, rmIntegrin alpha 4, recombinant human (rh) Integrin alpha M, and rhIntegrin alpha V is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha E/CD103
    Phe20-Arg1113 (Ser453Gly)
    Accession # ABD49099
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Integrin  alpha E/CD103 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Goat Anti-Mouse Integrin  alpha E/CD103 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1990G) and Rat Anti-Mouse CD8 alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB116A). Quadrant markers were set based on control antibody staining (Catalog # IC108G). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha E/CD103
The Integrin  alpha E subunit, also known as CD103, forms a non-covalent heterodimer with Integrin  beta 7. Integrin  alpha E/ beta 7 is a receptor for E-Cadherin. Integrin  alpha E is expressed in intra-epithelial lymphocytes and mediates adhesion of intra-epithelial lymphocytes to epithelial cell monolayers.
  • Entrez Gene IDs:
    3682 (Human); 16407 (Mouse); 83577 (Rat)
  • Alternate Names:
    antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103 antigen; CD103; HML-1 antigen; HUMINAE; integrin alpha-E; Integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide); ITGAE; MGC141996; Mucosal lymphocyte 1 antigen
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Alexa Fluor® 488-conjugated Control

Ctrl IC108G
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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