Mouse Integrin alpha E/CD103 Alexa Fluor® 488-conjugated Antibody

Flow Cytometry

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha E/CD103 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 2, rmIntegrin alpha 3, rmIntegrin alpha 4, recombinant human (rh) Integrin alpha M, and rhIntegrin alpha V is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha E/CD103
Phe20-Arg1113 (Ser453Gly)
Accession # ABD49099
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended
Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry

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Detection of Integrin alpha E/CD103 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Goat Anti-Mouse Integrin alpha E/CD103 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1990G) and Rat Anti-Mouse CD8 alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB116A). Quadrant markers were set based on control antibody staining (Catalog # IC108G). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha E/CD103

The Integrin alpha E subunit, also known as CD103, forms a non-covalent heterodimer with Integrin beta 7. Integrin alpha E/ beta 7 is a receptor for E-Cadherin. Integrin alpha E is expressed in intra-epithelial lymphocytes and mediates adhesion of intra-epithelial lymphocytes to epithelial cell monolayers.

Entrez Gene IDs:
3682 (Human); 16407 (Mouse); 83577 (Rat)
Alternate Names:
antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103 antigen; CD103; HML-1 antigen; HUMINAE; Integrin alpha E; integrin alpha-E; Integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide); ITGAE; MGC141996; Mucosal lymphocyte 1 antigen

Related Research Areas

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Isotype Controls

Description Application Cat# Citations Images

Normal Goat IgG Alexa Fluor® 488-conjugated Control

Ctrl IC108G

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Staining Reagents

Description Application Cat# Citations Images

Flow Cytometry Staining Buffer (1X)

Flow FC001 6

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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4

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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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