Mouse Integrin alpha E/CD103 Antibody

R&D Systems | Catalog # AF1990

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, CyTOF-reported

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Integrin alpha E/CD103 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha E/CD103
Phe20-Arg1113
Accession # Q60677

Specificity

Detects mouse Integrin alpha E/CD103 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Integrin alpha E/CD103 Antibody

Detection of Integrin  alpha E/CD103 in Mouse splenocytes by Flow Cytometry

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Goat Anti-Mouse Integrin  alpha E/CD103 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1990) or (B) isotype control antibody (Catalog # AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse Integrin alpha E/CD103 by Immunohistochemistry

Detection of Mouse Integrin alpha E/CD103 by Immunohistochemistry

AXL upregulation in TKI-resistant HCC inversely correlates with tumor infiltration of immune cells and may predict treatment response. A Lists of upregulated druggable targets in sorafenib-resistant HepG2 cells and lenvatinib-resistant HCC patient-derived xenograft (PDX). The numbers and the color scale bar indicate the expression fold-change of resistant samples versus sensitive samples. B Bubble plots showing the correlation analysis of AXL with immune gene signatures in sorafenib-resistant samples from the datasets GSE176151 (left) and GSE151412 (right). The color scale bar and the size of the dots indicate the Pearson correlation coefficient. C Representative multiplex immunofluorescence images (top) of staining of CD103, CD8 alpha, AXL, and DAPI in residual xenografts after treatment with DMOS, Sora, or Lenva. Scale bar = 50 µm. D Immunotherapy response prediction (top) of TCGA-LIHC patients using Tumor immune dysfunction and exclusion (TIDE) analysis. Heatmap (bottom) of TIDE scores and the corresponding AXL expression (normalized count) of patients from TCGA-LIHC dataset. E Violin plot of AXL expression in immunotherapy responders and non-responders as predicted by TIDE. F Heatmap showing AXL expression, T-cell dysfunction score, and T-cell exclusion score of patients from TCGA-LIHC dataset. G Violin plots of T-cell dysfunction score (left) and T-cell exclusion score (right) in AXL-high and AXL-low patients. *p < 0.05; **p < 0.01; ***p < 0.001 on a two-tailed unpaired Student’s t test. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41419-024-06493-0), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Integrin alpha E/CD103 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: See below

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Integrin  alpha E/CD103

Reviewed Applications

Read 1 review rated 5 using AF1990 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha E/CD103

The alpha E subunit, also known as CD103, forms a non-covalent heterodimer with Integrin  beta 7. Integrin  alpha E/ beta 7 is a receptor for E-Cadherin. Integrin  alpha E is expressed in intra‑epithelial lymphocytes and mediates adhesion of intra‑epithelial lymphocytes to epithelial cell monolayers.

Alternate Names

CD103, HUMINAE, ITGAE

Entrez Gene IDs

3682 (Human); 16407 (Mouse); 83577 (Rat)

Gene Symbol

ITGAE

UniProt

Q60677

Additional Integrin alpha E/CD103 Products

Product Documents for Mouse Integrin alpha E/CD103 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Integrin alpha E/CD103 Antibody

For research use only

Citations for Mouse Integrin alpha E/CD103 Antibody

Customer Reviews for Mouse Integrin alpha E/CD103 Antibody (1)

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  • Mouse Integrin  alpha E/CD103 Antibody
    Name: Efstathios Stamatiades
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult small intestine
    Species: Mouse
    Verified Customer | Posted 07/09/2019
    Cryosections from PLP immersion fixed tissue were stained for Itgae (1:100) for 4h @ RT. After washing, tissue was stained with Donkey anti-Goat alexa546 2ndary antibody for 1h at RT. CD3 (green), Itgae (red), Hoechst (blue).
    Mouse Integrin alpha E/CD103 Antibody AF1990

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