Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Integrin alpha E/CD103 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin  alpha E
Phe20-Arg1113 (Ser453Gly)
Accession # ABD49099

Specificity

Detects mouse Integrin alpha E/CD103 in Western blots. In Western blots, less than 2% cross‑reactivity with recombinant mouse (rm) Integrin  alpha 2, rmIntegrin  alpha 3, rmIntegrin alpha 4, rmIntegrin alpha M, and recombinant human Integrin alpha V is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Integrin  alpha E/CD103 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Integrin  alpha E/CD103

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha E/CD103

The alpha E subunit, also known as CD103, forms a non-covalent heterodimer with Integrin  beta 7. Integrin  alpha E/ beta 7 is a receptor for E-Cadherin. Integrin  alpha E is expressed in intra‑epithelial lymphocytes and mediates adhesion of intra‑epithelial lymphocytes to epithelial cell monolayers.

Alternate Names

CD103, HUMINAE, ITGAE

Entrez Gene IDs

3682 (Human); 16407 (Mouse); 83577 (Rat)

Gene Symbol

ITGAE

UniProt

ABD49099

Additional Integrin alpha E/CD103 Products

Product Documents for Mouse Integrin  alpha E/CD103 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Integrin  alpha E/CD103 Biotinylated Antibody

For research use only

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