Mouse Integrin beta 4/CD104 PE-conjugated Antibody

Name: Mouse Integrin beta 4/CD104 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB4054P

Catalog #: FAB4054P Datasheet / COA / SDS

Discontinued Product

FAB4054P has been discontinued.

View all Integrin beta 4/CD104 products.

Detection of Integrin beta 4/CD104 in CMT‑93 Mouse Cell Line by Flow Cytometry.

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All Integrin beta 4/CD104 Antibodies

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Mouse Integrin beta 4/CD104 PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin beta 4/CD104 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse Integrin beta 1, beta 2, beta 6, beta 7, or recombinant human Integrin beta 3.

Source

Monoclonal Rat IgG_2A Clone # 308601

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 4/CD104
Asn29-Ser711
Accession # NP_001005608

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin beta 4/CD104 in CMT‑93 Mouse Cell Line by Flow Cytometry. CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse Integrin beta 4/CD104 PE-conjugated Monoclonal Antibody (Catalog # FAB4054P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin beta 4/CD104

Integrin beta 4, also known as CD104, is a 200 kDa type I transmembrane (TM) glycoprotein that associates only with Integrin alpha 6/CD29. Integrin alpha 6 beta 4 is predominantly expressed by epithelial cells, binds laminins, and is essential for formation of hemidesmosomes and connection of the dermis to the epidermis. Mouse integrin beta 4 contains a 680 amino acid (aa) extracellular domain (ECD) with a metal-binding site and four cysteine-rich repeats, a 22 aa TM segment and an unusually long 1073 aa cytoplasmic tail that organizes hemidesmosome components. Isoforms with shortened cytoplasmic tails have been described. Mouse and human beta 4 ECD show 88% aa identity.

Entrez Gene IDs

3691 (Human); 192897 (Mouse); 25724 (Rat)

Alternate Names

CD104 antigen; CD104; GP150; Integrin beta 4; integrin beta-4 subunit; integrin beta-4; integrin, beta 4; ITGB4