Mouse Integrin  beta 4/CD104 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse Integrin beta 4/CD104 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse Integrin beta 1, beta 2, beta 6, beta 7, or recombinant human Integrin beta 3.
  • Source
    Monoclonal Rat IgG2A Clone # 308601
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin  beta 4/CD104
    Accession # NP_001005608
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Integrin  beta 4/CD104 in
CMT‑93 Mouse Cell Line by Flow Cytometry.
CMT‑93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse Integrin  beta 4/CD104 PE‑conjugated Monoclonal Antibody (Catalog # FAB4054P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin beta 4/CD104
Integrin beta 4, also known as CD104, is a 200 kDa type I transmembrane (TM) glycoprotein that associates only with Integrin  alpha 6/CD29. Integrin alpha 6 beta 4 is predominantly expressed by epithelial cells, binds laminins, and is essential for formation of hemidesmosomes and connection of the dermis to the epidermis. Mouse integrin beta 4 contains a 680 amino acid (aa) extracellular domain (ECD) with a metal-binding site and four cysteine-rich repeats, a 22 aa TM segment and an unusually long 1073 aa cytoplasmic tail that organizes hemidesmosome components. Isoforms with shortened cytoplasmic tails have been described. Mouse and human beta 4 ECD show 88% aa identity.
  • Entrez Gene IDs:
    3691 (Human); 192897 (Mouse); 25724 (Rat)
  • Alternate Names:
    CD104 antigen; CD104; GP150; integrin beta-4 subunit; integrin beta-4; integrin, beta 4; ITGB4
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 13  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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