Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Intelectin-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Intelectin-2
Ala20-Ser298
Accession # Q80ZA0

Specificity

Detects mouse Intelectin-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant mouse Intelectin-1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Intelectin‑2 Antibody

Detection of Mouse Intelectin‑2 antibody by Western Blot.

Detection of Mouse Intelectin‑2 by Western Blot.

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Intelectin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5358) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Intelectin-2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse Intelectin‑2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse small intestine tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Intelectin-2

Intelectin-2 (ITLN-2, ITLN-1b and HL-2) is a 37-41 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by airway mucous cells plus intestinal Paneth and goblet cells, and appears to participate in fungal microbe recognition. The mouse ITLN-2 precursor is 313 amino acids (aa) in length. It contains a 19 aa signal sequence, a 279 aa mature region (aa 20-298), and a GPI-linked prodomain (aa 299-313). The mature region contains one C-terminal type fibrinogen domain (aa 32-251). Over aa 20-298, mouse ITLN-2 shares 79% aa sequence identity with human ITLN-2, and 92% aa sequence identity with mouse ITLN-1.

Alternate Names

HL-2, Intelectin-b, Intelectin2, Itln1b, ITLN2, Itlnb

Entrez Gene IDs

142683 (Human); 493583 (Mouse)

Gene Symbol

ITLN2

UniProt

Q80ZA0

Additional Intelectin-2 Products

Product Documents for Mouse Intelectin‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Intelectin‑2 Antibody

For research use only

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