Mouse JAM-4/IGSF5 Antibody

Catalog # Availability Size / Price Qty
AF3836
AF3836-SP
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Mouse JAM-4/IGSF5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse JAM‑4/IGSF5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) JAM-A, rmJAM-B, and rmJAM-C is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse JAM‑4/IGSF5
Leu15-Ala139
Accession # NP_082354
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse JAM‑4/IGSF5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAM-4/IGSF5

JAM-4, also known as IGSF5, is a 93 kDa member of the CTX (Cortical Thymocyte Marker in Xenopus) subfamily of the Ig superfamily. It is a type I transmembrane protein with an N-terminal extracellular V-type and C2 type Ig domain separated by a J segment, a transmembrane domain and a cytoplasmic tail with a PDZ binding motif. JAM-4 is expressed in intestinal, renal, and mammary epithelial cells, spermatogonia, and hematopoietic stem cells. It is a cell adhesion molecule that mediates homophilic interaction. JAM-4 also interacts with the intracellular membrane-associated guanylate kinase inverted 1 (MAGI-1). An additional isoform containing a 100 residue insertion between Gln 125 and Asp 126 has been reported. Over the region used as immunogen, mouse JAM-4 shares 82% and 63% amino acid sequence identity with rat and human JAM-4, respectively.

Long Name
Junctional Adhesion Molecule 4
Entrez Gene IDs
150084 (Human); 72058 (Mouse)
Alternate Names
GSF5; IGSF5; Immunoglobulin Superfamily 5 Like; Immunoglobulin Superfamily Member 5; Immunoglobulin Superfamily, Member 5; JAM4; JAM-4; JCAM; Junctional Adhesion Molecule 4; Putative Gene, Immunoglobulin Superfamily 5 Like11

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