Mouse JAM‑A Alexa Fluor® 532‑conjugated Antibody

Name: Mouse JAM‑A Alexa Fluor® 532‑conjugated Antibody
Brand: R&D Systems
SKU: AF1077X
Price: 549 USD
Availability: InStock

R&D Systems | Catalog # AF1077X

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Product Documents

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair)

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Polyclonal Goat IgG

View all JAM-A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse JAM-A
Lys27-Ala242
Accession # O88792

Specificity

Detects mouse JAM‑A in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross‑reactivity with recombinant human JAM‑A, recombinant mouse (rm) JAM‑B, and rmJAM‑C is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse JAM‑A Alexa Fluor® 532‑conjugated Antibody

Application

Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilution of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: JAM-A

The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-A is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (3). It is also expressed on circulating megakaryocytes. Mouse JAM-A cDNA predicts a 300 amino acid (aa) residue precursor protein with a putative 23 aa signal peptide, a 215 aa extracellular region containing two Ig-like V-subset domains, a 17 aa transmembrane domain and a 45 aa cytoplasmic domain. The human and mouse protein share approximately 67% aa sequence homology. Mouse JAM-A also shares approximately 35% aa sequence homology with mouse JAM-B or JAM-C. JAM-A exhibits homotypic interactions to regulate tight junction assembly and modulate paracellular permeability (1‑3). The human JAM-A homotypic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (4). JAM-A is involved in leukocyte adhesion and transmigration through the endothelium (3, 5). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (6).

Long Name

Junctional Adhesion Molecule A

Alternate Names

CD321, F11R, JAMA, PAM-1

Entrez Gene IDs

50848 (Human); 16456 (Mouse); 101925695 (Cynomolgus Monkey)

Gene Symbol

F11R

UniProt

O88792

Additional JAM-A Products

Product Documents for Mouse JAM‑A Alexa Fluor® 532‑conjugated Antibody

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Product Specific Notices for Mouse JAM‑A Alexa Fluor® 532‑conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only