Mouse JAM-A Antibody

Name: Mouse JAM-A Antibody
Brand: R&D Systems
SKU: AF1077
Price: 189 USD
Availability: InStock

R&D Systems | Catalog # AF1077

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Citations (25)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all JAM-A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse JAM-A
Lys27-Ala242
Accession # O88792

Specificity

Detects mouse JAM‑A in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross‑reactivity with recombinant human JAM‑A, recombinant mouse (rm) JAM‑B, and rmJAM‑C is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse JAM-A Antibody

Mouse JAM-A ELISA Standard Curve

Recombinant Mouse JAM‑A Fc Chimera (Catalog # 1077-JM) was serially diluted and captured by Goat Anti-Mouse JAM‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1077) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse JAM‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1077) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Mouse JAM-A ELISA Standard Curve

Detection of JAM-A by Immunohistochemistry

Lactobacillus johnsonii N5 improves the intestinal barrier tight junction protein and HSP70 expressions in dextran sulfate sodium-induced colitis. (A) Representative images of colonic tissue sections stained with antibodies specific for ZO-1 (red), occludin (yellow), and JAM-A (white). Sections were counterstained with Nucleus (blue). Scale bars = 50 μm. (B to D) The expression of ZO-1, occludin, and JAM-A was quantified as mean fluorescence intensity (MFI). (E) Representative images of colonic tissue sections stained with anti-HSP70 (green) antibody. Sections were counterstained with Nucleus (blue). Scale bars = 50 μm. (F) Analysis and quantification of HSP70 expression, n = 6 mice per group (duplicate slides per mouse). Control, mice were fed a commercial chow diet and received vehicle treatment; DSS, mice were given 2.5% of dextran sulfate sodium in drinking water for 7 consecutive days; N5, mice were administered L. johnsonii N5 daily for 7 days; N5-DSS, mice were administered L. johnsonii N5 daily for 14 days starting 7 days prior to DSS-treatment. ZO-1 = zonula occludens protein 1; JAM-A = junctional adhesion molecule A; MFI = mean fluorescence intensity; HSP70 = heat shock proteins 70. Data are presented as mean ± SEM, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, using ANOVA with Tukey's post hoc test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38033603), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse JAM-A Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse small intestine

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse JAM-A Fc Chimera (Catalog # 1077-JM)

Mouse JAM-A Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse JAM‑A Biotinylated Antibody (Catalog # BAF1077)
Standard: Recombinant Mouse JAM-A Fc Chimera Protein, CF (Catalog # 1077-JM)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: JAM-A

The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-A is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (3). It is also expressed on circulating megakaryocytes. Mouse JAM-A cDNA predicts a 300 amino acid (aa) residue precursor protein with a putative 23 aa signal peptide, a 215 aa extracellular region containing two Ig-like V-subset domains, a 17 aa transmembrane domain and a 45 aa cytoplasmic domain. The human and mouse protein share approximately 67% aa sequence homology. Mouse JAM-A also shares approximately 35% aa sequence homology with mouse JAM-B or JAM-C. JAM-A exhibits homotypic interactions to regulate tight junction assembly and modulate paracellular permeability (1‑3). The human JAM-A homotypic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (4). JAM-A is involved in leukocyte adhesion and transmigration through the endothelium (3, 5). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (6).