Mouse JAM-A Antibody

Catalog # Availability Size / Price Qty
AF1077
AF1077-SP

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Product Details
Citations (7)
FAQs
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Mouse JAM-A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse JAM‑A in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross‑reactivity with recombinant human JAM‑A, recombinant mouse (rm) JAM‑B, and rmJAM‑C is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse JAM-A
Lys27-Ala242
Accession # O88792
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse JAM-A Fc Chimera (Catalog # 1077-JM)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse small intestine

Mouse JAM-A Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse JAM‑A Biotinylated Antibody (Catalog # BAF1077)

Standard: Recombinant Mouse JAM-A Fc Chimera Protein, CF (Catalog # 1077-JM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAM-A

The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-A is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (3). It is also expressed on circulating megakaryocytes. Mouse JAM-A cDNA predicts a 300 amino acid (aa) residue precursor protein with a putative 23 aa signal peptide, a 215 aa extracellular region containing two Ig-like V-subset domains, a 17 aa transmembrane domain and a 45 aa cytoplasmic domain. The human and mouse protein share approximately 67% aa sequence homology. Mouse JAM-A also shares approximately 35% aa sequence homology with mouse JAM-B or JAM-C. JAM-A exhibits homotypic interactions to regulate tight junction assembly and modulate paracellular permeability (1‑3). The human JAM-A homotypic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (4). JAM-A is involved in leukocyte adhesion and transmigration through the endothelium (3, 5). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (6).

References
  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Martin-Padura, I. et al. (1998) J. Cell Biol. 142:117.
  4. Babinska, A. et al. (2002) Thromb. Haemost. 88:842.
  5. Del Maschio, A. et al. (1999) J. Exp. Med. 190:1351.
  6. Barton, E. S. et al. (2001) Cell 104:441.
Long Name
Junctional Adhesion Molecule A
Entrez Gene IDs
50848 (Human); 16456 (Mouse); 101925695 (Cynomolgus Monkey)
Alternate Names
CD321 antigen; CD321; F11 receptor; F11R; JAM-1; JAM1CD321; JAMA; JAM-A; JCAMJAM; Junctional adhesion molecule 1Platelet F11 receptor; junctional adhesion molecule A; PAM-1; PAM-1KAT; Platelet adhesion molecule 1

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Citations for Mouse JAM-A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Macrophage-dependent neutrophil recruitment is impaired under conditions of increased intestinal permeability in JAM-A-deficient mice
    Authors: AC Luissint, HC Williams, W Kim, S Flemming, V Azcutia, RS Hilgarth, MNO Leary, TL Denning, A Nusrat, CA Parkos
    Mucosal Immunol, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Analysis of leukocyte transepithelial migration using an in vivo murine colonic loop model
    Authors: S Flemming, AC Luissint, A Nusrat, CA Parkos
    JCI Insight, 2018;3(20):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Dysregulation of junctional adhesion molecule-A contributes to ethanol-induced barrier disruption in intestinal epithelial cell monolayers
    Authors: DM Chopyk, P Kumar, R Raeman, Y Liu, T Smith, FA Anania
    Physiol Rep, 2017;5(23):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis
    Authors: E Hintermann, M Bayer, J Ehser, M Aurrand-Li, JM Pfeilschif, BA Imhof, U Christen
    Cell Adh Migr, 2016;10(4):419-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells.
    Authors: Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T
    Mol Cancer, 2015;14(0):39.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Regulated release and functional modulation of junctional adhesion molecule A by disintegrin metalloproteinases.
    Authors: Koenen RR, Pruessmeyer J, Soehnlein O, Fraemohs L, Zernecke A, Schwarz N, Reiss K, Sarabi A, Lindbom L, Hackeng TM, Weber C, Ludwig A
    Blood, 2009;113(19):4799-809.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  7. JAM-A is present in mammalian spermatozoa where it is essential for normal motility.
    Authors: Shao M, Ghosh A, Cooke VG, Naik UP, Martin-DeLeon PA
    Dev. Biol., 2007;313(1):246-55.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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