Mouse JAM-A Biotinylated Antibody

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Product Details
Citations (1)
Supplemental Products

Mouse JAM-A Biotinylated Antibody Summary

Species Reactivity
Detects mouse JAM-A in ELISAs in Western blots. In sandwich immunoassays, less than 1% cross-reactivity is observed with recombinant human JAM-A, recombinant mouse (rm) JAM-B, and rmJAM-C.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse JAM-A
Accession # O88792
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse JAM-A Fc Chimera (Catalog # 1077-JM)

Mouse JAM-A Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse JAM‑A Antibody (Catalog # AF1077)

Standard: Recombinant Mouse JAM-A Fc Chimera Protein, CF (Catalog # 1077-JM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAM-A

The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-A is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (3). It is also expressed on circulating megakaryocytes. Mouse JAM-A cDNA predicts a 300 amino acid (aa) residue precursor protein with a putative 23 aa signal peptide, a 215 aa extracellular region containing two Ig-like V-subset domains, a 17 aa transmembrane domain and a 45 aa cytoplasmic domain. The human and mouse protein share approximately 67% aa sequence homology. Mouse JAM-A also shares approximately 35% aa sequence homology with mouse JAM-B or JAM-C. JAM-A exhibits homotypic interactions to regulate tight junction assembly and modulate paracellular permeability (1‑3). The human JAM-A homotypic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (4). JAM-A is involved in leukocyte adhesion and transmigration through the endothelium (3, 5). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (6).

  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Martin-Padura, I. et al. (1998) J. Cell Biol. 142:117.
  4. Babinska, A. et al. (2002) Thromb. Haemost. 88:842.
  5. Del Maschio, A. et al. (1999) J. Exp. Med. 190:1351.
  6. Barton, E. S. et al. (2001) Cell 104:441.
Long Name
Junctional Adhesion Molecule A
Entrez Gene IDs
50848 (Human); 16456 (Mouse); 101925695 (Cynomolgus Monkey)
Alternate Names
CD321 antigen; CD321; F11 receptor; F11R; JAM-1; JAM1CD321; JAMA; JAM-A; JCAMJAM; Junctional adhesion molecule 1Platelet F11 receptor; junctional adhesion molecule A; PAM-1; PAM-1KAT; Platelet adhesion molecule 1

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Citation for Mouse JAM-A Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Regulated release and functional modulation of junctional adhesion molecule A by disintegrin metalloproteinases.
    Authors: Koenen RR, Pruessmeyer J, Soehnlein O, Fraemohs L, Zernecke A, Schwarz N, Reiss K, Sarabi A, Lindbom L, Hackeng TM, Weber C, Ludwig A
    Blood, 2009-03-03;113(19):4799-809.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development


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