Mouse JAM-B/VE-JAM Antibody (150015)

Name: Mouse JAM-B/VE-JAM Antibody (150015)
Brand: R&D Systems
SKU: MAB988
Price: 569 USD
Availability: InStock

R&D Systems | Catalog # MAB988

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Citations (3)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG₁ Clone # 150015

View all JAM-B/VE-JAM Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse JAM‑B/VE-JAM
Phe29-Asn236 (predicted)
Accession # Q9JI59

Specificity

Detects mouse JAM‑B/VE-JAM in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human JAM-B/VE-JAM is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse JAM-B/VE-JAM Antibody (150015)

Cell Adhesion Mediated by JAM‑B/VE‑JAM and Neutral-ization by Mouse JAM‑B/ VE‑JAM Antibody.

Recombinant Mouse JAM-B/VE-JAM Fc Chimera (Catalog # 988-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse JAM-B/VE-JAM Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse JAM-B/ VE-JAM Monoclonal Antibody (Catalog # MAB988). The ND₅₀ is typically 0.1-0.5 µg/mL.

Detection of cm48lzo2cyr2ggmnee5f.jpg by Western Blot

The cleaved JAM-C ectodomain is accumulated in the fat interstitial tissues. (A) Schematic illustration for detecting full-length JAM-C (fJAM-C) and/or soluble JAM-C (sJAM-C) using JAM-C (N) and JAM-C (C) antibodies (Abs) targeting the N- and C-termini, respectively. (B) Knockdown of the Jam3 gene encoding mouse JAM-C in ADSCs using the CRISPR method. (C) Western blot for the indicated proteins in the whole-cell lysates and supernatants of the revealed ADSCs. (D) Western blot for the indicated proteins in the whole-cell lysates of mouse adult spleen tissue (control), adipose tissue and ADSCs. (E) Confocal images of mouse adipose tissue stained with JAM-C (N) and JAM-C (C) Abs. Asterisks indicate lipid droplets in differentiated adipocytes. Squares show the enlarged areas. Scale bar, 50 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33801826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of iz3qwgav7zcxfwh7li0e.jpg by Flow Cytometry

Fluorescence-activated cell sorting (FACS) profiles of the stromal vascular fraction (SVF) of the mouse adipose tissue. (A) Association between the expression of mesenchymal stem cell (MSC) markers CD44, CD105, CD140a and Sca-1 and JAM-B or JAM-C expression. (B) The JAM-C expression in the Sca1 /CD31−/CD45−/Ter119− cell lineage. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33801826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of JAM-B/VE-JAM by Western Blot

A Soluble JAM-C couples with JAM-B to promote the ADSC adhesion and niche function. (A) Knockdown of the Jam2 gene encoding mouse JAM-B in ADSCs using the CRISPR method. (B) Western blot for the indicated proteins in the whole-cell lysates of the revealed ADSCs. (C) Cell adhesion assay for ADSCs grown in the indicated culture conditions. The relative levels are shown in histograms (mean ± SD; n = 8). (D) RT-qPCR for the indicated MSC markers in ADSCs cultivated in the indicated culture conditions. The relative expression levels are shown in the histograms (mean ± SD; n = 4). * p < 0.05; ** p < 0.01; *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33801826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of JAM-B/VE-JAM by Immunocytochemistry/ Immunofluorescence

JAM-B and JAM-C are concentrated on cell membranes of mouse adipose-derived stromal/stem cells (ADSCs). RT-PCR (A) and Western blot (B) for the indicated molecules in ADSCs derived from three mice (#1–3). Mouse kidney (for Jam-4) and spleen tissues (for other junctional adhesion molecules; JAMs) are used as positive controls (Ctrl). P, passage. (C) Confocal images of ADSCs stained for the indicated markers. Arrowheads show JAM-B- and JAM-C-immunoreactive signals on the cell membranes of ADSCs. Squares indicate the enlarged areas. Scale bar, 50 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33801826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of JAM-B/VE-JAM by Western Blot

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Applications for Mouse JAM-B/VE-JAM Antibody (150015)

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse JAM-B/VE-JAM Fc Chimera (Catalog # 988-VJ)

Neutralization

Measured by its ability to neutralize JAM‑B/VE‑JAM-mediated adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line. Fong, S. et al. (2002) J. Immunol. 168:1618. The Neutralization Dose (ND₅₀) is typically 0.1-0.5 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse JAM‑B/VE‑JAM Fc Chimera.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: JAM-B/VE-JAM

The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. JAM-B, alternatively named vascular endothelial JAM (VE-JAM), is expressed prominently on high endothelial venules of lymphoid organs where it is localized to the intercellular boundaries of high endothelial cells. It is also expressed on the endothelium of a variety of non-lymphoid organs, especially the heart and placenta (2, 3, 5). Mouse JAM-B/VE-JAM cDNA predicts a 298 amino acid (aa) precursor protein with a putative 28 aa signal peptide, a 209 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 38 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (2, 3). Mouse JAM-B shares approximately 79% aa sequence homology with its human homologue. It also shares approximately 35% aa sequence homology with mouse JAM-A or JAM‑C. JAM-B exhibits homotypic interactions, as well as heterotypic interactions with JAM‑C, but not JAM-A (4, 5, 7). It is also a ligand for the Integrin alpha4beta1. However, the JAM-B/alpha4beta1 interaction is facilitated only after prior adhesion of JAM-B to JAM‑C (6). Through its heterotypic interactions with JAM‑C, JAM-B is an adhesive ligand for T, NK, and dendritic cells, and may play a role in regulating leukocyte transmigration (5).