Mouse JAM-C Antibody Summary
Accession # Q9D8B7
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
JAM-C in Mouse Embryo. JAM-C was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
JAM‑C in Mouse Splenocytes. JAM-C was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The family of junctional adhesion molecules (JAM), comprised of at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-C cDNA predicts a 310 amino acid (aa) residue precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, an 18 aa transmembrane domain and a 51 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3). Mouse JAM-C shares 86% aa sequence identity with its human homologue. It also shares approximately 31% and 35% aa sequence homology with mouse JAM-A and JAM-B, respectively (2). Mouse JAM-C is highly expressed during embryogenesis. In adult tissues, mouse JAM-C is restricted to endothelial cells, lymph endothelial cells in the kidney, lymph node and Peyer’s patches where the protein can be localized to the high endothelial venules (3). Although human JAM-C is expressed on human platelets and a subset of leukocytes, mouse JAM-C expression was not detected on any mouse lymphocytes (4). In contrast to human JAM-C which show weak homotypic interactions, mouse JAM-C was reported to exhibit homotypic interactions (3). Mouse JAM-C has also been shown to have heterotypic interaction with JAM-B. It is likely that mouse JAM-C may play a role in lymphocyte transendothelial migration (4).
The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.
- Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
- Aurrand-Lions, M. et al. (2001) Blood 98:3699.
- Aurrand-Lions, M. et al. (2001) J. Biol. Chem. 276:2733.
- Johnson-Leger, C. et al. (2002) Blood 100:25793.
Citations for Mouse JAM-C Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis
Authors: E Hintermann, M Bayer, J Ehser, M Aurrand-Li, JM Pfeilschif, BA Imhof, U Christen
Cell Adh Migr, 2016;10(4):419-33.
Sample Types: Whole Cells
Applications: Flow Cytometry
Junctional adhesion molecule (JAM)-C deficient C57BL/6 mice develop a severe hydrocephalus.
Authors: Wyss L, Schafer J, Liebner S, Mittelbronn M, Deutsch U, Enzmann G, Adams R, Aurrand-Lions M, Plate K, Imhof B, Engelhardt B
PLoS ONE, 2012;7(9):e45619.
Sample Types: Whole Tissue
Expression, localization, and function of junctional adhesion molecule-C (JAM-C) in human retinal pigment epithelium.
Authors: Economopoulou M, Hammer J, Wang F, Fariss R, Maminishkis A, Miller SS
Invest. Ophthalmol. Vis. Sci., 2009;50(3):1454-63.
Sample Types: Whole Cells
Junctional adhesion molecule-C promotes metastatic potential of HT1080 human fibrosarcoma.
Authors: Fuse C, Ishida Y, Hikita T, Asai T, Oku N
J. Biol. Chem., 2007;282(11):8276-83.
Sample Types: Cell Lysates
Applications: Western Blot
Junctional adhesion molecules (JAM)-B and -C contribute to leukocyte extravasation to the skin and mediate cutaneous inflammation.
Authors: Ludwig RJ, Zollner TM, Santoso S, Hardt K, Gille J, Baatz H, Johann PS, Pfeffer J, Radeke HH, Schon MP, Kaufmann R, Boehncke WH, Podda M
J. Invest. Dermatol., 2005;125(5):969-76.
Sample Types: In Vivo
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