Mouse JAM-C Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse JAM-C in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human JAM‑C and less than 2% cross-reactivity with recombinant mouse (rm) JAM-A and rmJAM-B is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse JAM-C
    Val32-Asn241
    Accession # Q9D8B7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse JAM-C Fc Chimera (Catalog # 1213-J3)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 3-15 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of biotinylated Recombinant Mouse VEJAM to immobilized Recombinant Mouse JAM-C (Catalog # 1213-J3) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
JAM-C in Mouse Embryo. JAM-C was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunocytochemistry
JAM‑C in Mouse Splenocytes. JAM‑C was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse JAM‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: JAM-C

The family of junctional adhesion molecules (JAM), comprised of at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-C cDNA predicts a 310 amino acid (aa) residue  precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, an 18 aa transmembrane domain and a 51 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3). Mouse JAM-C shares 86% aa sequence identity with its human homologue. It also shares approximately 31% and 35% aa sequence homology with mouse JAM-A and JAM-B, respectively (2). Mouse JAM-C is highly expressed during embryogenesis. In adult tissues, mouse JAM-C is restricted to endothelial cells, lymph endothelial cells in the kidney, lymph node and Peyer’s patches where the protein can be localized to the high endothelial venules (3). Although human JAM-C is expressed on human platelets and a subset of leukocytes, mouse JAM-C expression was not detected on any mouse lymphocytes (4). In contrast to human JAM-C which show weak homotypic interactions, mouse JAM-C was reported to exhibit homotypic interactions (3). Mouse JAM-C has also been shown to have heterotypic interaction with JAM-B. It is likely that mouse JAM-C may play a role in lymphocyte transendothelial migration (4).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.

  • References:
    1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
    2. Aurrand-Lions, M. et al. (2001) Blood 98:3699.
    3. Aurrand-Lions, M. et al. (2001) J. Biol. Chem. 276:2733.
    4. Johnson-Leger, C. et al. (2002) Blood 100:25793.
  • Long Name:
    Junctional Adhesion Molecule C
  • Entrez Gene IDs:
    83700 (Human); 83964 (Mouse)
  • Alternate Names:
    CD323; JAM-2; JAM3; JAMC; JAM-C; JAM-CFLJ14529; junctional adhesion molecule 3JAM-3; junctional adhesion molecule C
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Junctional adhesion molecule (JAM)-C deficient C57BL/6 mice develop a severe hydrocephalus.
    Authors: Wyss L, Schafer J, Liebner S, Mittelbronn M, Deutsch U, Enzmann G, Adams R, Aurrand-Lions M, Plate K, Imhof B, Engelhardt B
    PLoS ONE, 2012;7(9):e45619.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Expression, localization, and function of junctional adhesion molecule-C (JAM-C) in human retinal pigment epithelium.
    Authors: Economopoulou M, Hammer J, Wang F, Fariss R, Maminishkis A, Miller SS
    Invest. Ophthalmol. Vis. Sci., 2009;50(3):1454-63.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. Junctional adhesion molecule-C promotes metastatic potential of HT1080 human fibrosarcoma.
    Authors: Fuse C, Ishida Y, Hikita T, Asai T, Oku N
    J. Biol. Chem., 2007;282(11):8276-83.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Junctional adhesion molecules (JAM)-B and -C contribute to leukocyte extravasation to the skin and mediate cutaneous inflammation.
    Authors: Ludwig RJ, Zollner TM, Santoso S, Hardt K, Gille J, Baatz H, Johann PS, Pfeffer J, Radeke HH, Schon MP, Kaufmann R, Boehncke WH, Podda M
    J. Invest. Dermatol., 2005;125(5):969-76.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
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