Mouse JAM-C (CD323) APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7050A
Detection of JAM‑C (CD323) in B16‑F1 Mouse Cell Line by Flow Cytometry.
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Citations (2)
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Mouse JAM-C (CD323) APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse JAM-C (CD323) in flow cytometry.
Source
Monoclonal Rat IgG2B Clone # 209628
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse JAM‑C (CD323)
Val32-Asn241
Accession # Q9D8B7
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of JAM-C (CD323) antibody in B16-F1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of JAM‑C (CD323) in B16‑F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse JAM-C (CD323) APC-conjugated Monoclonal Antibody (Catalog # FAB7050A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: JAM-C

JAM-C (Junctional Adhesion Molecule-C), also known as JAM-3 and JAM-2, is a 40-45 kDa member of the JAM family, IgSF of molecules. It is a type I transmembrane glycoprotein that is further classified as a classical JAM with a short cytoplasmic tail vs. non-classical JAMs that contain long cytoplasmic tails. Mature mouse JAM-C is 289 amino acids (aa) in length, and contains a 212 aa extracellular region. This region possesses one N-terminal V-type and one C2-type Ig-like domain (aa 30-241). In human, JAM-C is perhaps best known as a component of the epithelial cell tight junction. In concert with two other transmembrane protein types (claudins and occludin) in-cis, JAM-C forms an intercellular barrier complex that restricts paracellular permeability. In mouse, however, this application may not apply, as endothelium, rather than epithelium, appears to be site of maximum expression. In this locale, JAM-C is suggested to regulate neutrophil exodus from the blood, and provide a barrier against reverse migration back into the vasculature. In any event, binding partners for JAM-C in-trans include JAM-B, Mac-1, alpha x beta 2 and JAM-C itself. Cells known to express JAM-C in mouse do not necessarily mirror those expressing JAM-C in human. Mouse cells known to contain JAM-C are limited in type and include endothelium, high endothelial venules, fibroblasts, hematopoietic stem cells, Schwann cells involved in myelination, and neurons of an Aδ (or pain sensing) phenotype. The extracellular domain of mouse JAM-C shares 96% and 87% aa sequence identity with the extracellular domains of rat and human JAM-C, respectively.

Long Name
Junctional Adhesion Molecule C
Entrez Gene IDs
83700 (Human); 83964 (Mouse)
Alternate Names
CD323; JAM-2; JAM3; JAMC; JAM-C; JAM-CFLJ14529; junctional adhesion molecule 3JAM-3; junctional adhesion molecule C

Product Datasheets

Citations for Mouse JAM-C (CD323) APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis
    Authors: V Radulovic, M van der Ga, S Koide, V Sigurdsson, S Lang, S Kaneko, K Miharada
    Cell Rep, 2019;27(10):2826-2836.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Function of Jam-B/Jam-C interaction in homing and mobilization of human and mouse hematopoietic stem and progenitor cells.
    Authors: Arcangeli M, Bardin F, Frontera V, Bidaut G, Obrados E, Adams R, Chabannon C, Aurrand-Lions M
    Stem Cells, 2014;32(4):1043-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse JAM-C (CD323) APC-conjugated Antibody

Average Rating: 4 (Based on 1 Review)

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Mouse JAM-C (CD323) APC-conjugated Antibody
By Jim Hsiao on 03/20/2018
Application: Flow Cytometry Sample Tested: Lung tissue Species: Mouse

Cells were pre-gated with live/dead and FSC-A/W to exclude dead cells and cell doublets.
The single cell suspension was isolated from lung of a WT B6 mouse and stained with the JAM-C (CD323) APC-conjugated antibody (1:100) for 30 minutes. Cells were analyzed by flow cytometer.