Mouse JAM-C (CD323) APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse JAM-C (CD323) in flow cytometry.
  • Source
    Monoclonal Rat IgG2B Clone # 209628
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse JAM‑C (CD323)
    Accession # Q9D8B7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of JAM‑C (CD323) in B16‑F1 Mouse Cell Line by Flow Cytometry. B16‑F1 mouse melanoma cell line was stained with Rat Anti-Mouse JAM‑C (CD323) APC‑conjugated Monoclonal Antibody (Catalog # FAB7050A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: JAM-C
JAM-C (Junctional Adhesion Molecule-C), also known as JAM-3 and JAM-2, is a 40-45 kDa member of the JAM family, IgSF of molecules. It is a type I transmembrane glycoprotein that is further classified as a classical JAM with a short cytoplasmic tail vs. non-classical JAMs that contain long cytoplasmic tails. Mature mouse JAM-C is 289 amino acids (aa) in length, and contains a 212 aa extracellular region. This region possesses one N-terminal V-type and one C2-type Ig-like domain (aa 30-241). In human, JAM-C is perhaps best known as a component of the epithelial cell tight junction. In concert with two other transmembrane protein types (claudins and occludin) in-cis, JAM-C forms an intercellular barrier complex that restricts paracellular permeability. In mouse, however, this application may not apply, as endothelium, rather than epithelium, appears to be site of maximum expression. In this locale, JAM-C is suggested to regulate neutrophil exodus from the blood, and provide a barrier against reverse migration back into the vasculature. In any event, binding partners for JAM-C in-trans include JAM-B, Mac-1, alpha x beta 2 and JAM-C itself. Cells known to express JAM-C in mouse do not necessarily mirror those expressing JAM-C in human. Mouse cells known to contain JAM-C are limited in type and include endothelium, high endothelial venules, fibroblasts, hematopoietic stem cells, Schwann cells involved in myelination, and neurons of an Aδ (or pain sensing) phenotype. The extracellular domain of mouse JAM-C shares 96% and 87% aa sequence identity with the extracellular domains of rat and human JAM-C, respectively.
  • Long Name:
    Junctional Adhesion Molecule C
  • Entrez Gene IDs:
    83700 (Human); 83964 (Mouse)
  • Alternate Names:
    CD323; JAM-2; JAM3; JAMC; JAM-CFLJ14529; junctional adhesion molecule 3JAM-3; junctional adhesion molecule C
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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