Mouse JAM‑C (CD323) APC‑conjugated Antibody

R&D Systems | Catalog # FAB7050A

R&D Systems
Discontinued Product
FAB7050A has been discontinued. View all JAM-C products.

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 209628
View all JAM-C Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse JAM‑C (CD323)
Val32-Asn241
Accession # Q9D8B7

Specificity

Detects mouse JAM-C (CD323) in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse JAM‑C (CD323) APC‑conjugated Antibody

Detection of JAM-C (CD323) antibody in B16-F1 Mouse Cell Line antibody by Flow Cytometry.

Detection of JAM‑C (CD323) in B16‑F1 Mouse Cell Line by Flow Cytometry.

B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse JAM-C (CD323) APC-conjugated Monoclonal Antibody (Catalog # FAB7050A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse JAM‑C (CD323) APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: B16‑F1 mouse melanoma cell line

Reviewed Applications

Read 3 reviews rated 4 using FAB7050A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: JAM-C

JAM-C (Junctional Adhesion Molecule-C), also known as JAM-3 and JAM-2, is a 40-45 kDa member of the JAM family, IgSF of molecules. It is a type I transmembrane glycoprotein that is further classified as a classical JAM with a short cytoplasmic tail vs. non-classical JAMs that contain long cytoplasmic tails. Mature mouse JAM-C is 289 amino acids (aa) in length, and contains a 212 aa extracellular region. This region possesses one N-terminal V-type and one C2-type Ig-like domain (aa 30-241). In human, JAM-C is perhaps best known as a component of the epithelial cell tight junction. In concert with two other transmembrane protein types (claudins and occludin) in-cis, JAM-C forms an intercellular barrier complex that restricts paracellular permeability. In mouse, however, this application may not apply, as endothelium, rather than epithelium, appears to be site of maximum expression. In this locale, JAM-C is suggested to regulate neutrophil exodus from the blood, and provide a barrier against reverse migration back into the vasculature. In any event, binding partners for JAM-C in-trans include JAM-B, Mac-1, alpha x beta 2 and JAM-C itself. Cells known to express JAM-C in mouse do not necessarily mirror those expressing JAM-C in human. Mouse cells known to contain JAM-C are limited in type and include endothelium, high endothelial venules, fibroblasts, hematopoietic stem cells, Schwann cells involved in myelination, and neurons of an A delta (or pain sensing) phenotype. The extracellular domain of mouse JAM-C shares 96% and 87% aa sequence identity with the extracellular domains of rat and human JAM-C, respectively.

Long Name

Junctional Adhesion Molecule C

Alternate Names

CD323, JAM3, JAMC

Entrez Gene IDs

83700 (Human); 83964 (Mouse)

Gene Symbol

JAM3

UniProt

Q9D8B7

Additional JAM-C Products

Product Documents for Mouse JAM‑C (CD323) APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse JAM‑C (CD323) APC‑conjugated Antibody

For research use only

Citations for Mouse JAM‑C (CD323) APC‑conjugated Antibody

Customer Reviews for Mouse JAM‑C (CD323) APC‑conjugated Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
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3 Stars
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  • Mouse JAM-C (CD323) APC-conjugated Antibody
    Name: James Xiao
    Application: Immunohistochemistry-Frozen
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 04/16/2018
    Purple: JAM_C Blue: DAPI+ nuclei
    Frozen mouse lung tissues of WT B6 mouse was blocked with 1% BSA in PBS and stained with APC-conjugated JAM-C antiboady (1:50) for 2 hours at the room temperature.
    Mouse JAM‑C (CD323) APC‑conjugated Antibody FAB7050A
  • Mouse JAM-C (CD323) APC-conjugated Antibody
    Name: Jim Hsiao
    Application: Flow Cytometry
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 03/20/2018
    Cells were pre-gated with live/dead and FSC-A/W to exclude dead cells and cell doublets. The single cell suspension was isolated from lung of a WT B6 mouse and stained with the JAM-C (CD323) APC-conjugated antibody (1:100) for 30 minutes. Cells were analyzed by flow cytometer.
    Mouse JAM‑C (CD323) APC‑conjugated Antibody FAB7050A
  • Mouse JAM-C (CD323) APC-conjugated Antibody
    Name: James Xiao
    Application: Flow Cytometry
    Sample Tested: Primary mouse lung endothelial cells
    Species: Mouse
    Verified Customer | Posted 09/08/2017
    The antibody was diluted at 1:100 with FACS buffer.
    Mouse JAM‑C (CD323) APC‑conjugated Antibody FAB7050A

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