Mouse Klotho  beta Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2619

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Citations (1)
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Mouse Klotho  beta Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Klotho beta in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse Klotho is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Klotho beta
Phe53-Leu995
Accession # NP_112457
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Klotho  beta (Catalog # 2619-KB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Klotho beta

Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1). Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex (2). The Klotho beta mediated interaction of FGF15 (human FGF19) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) (2‑4). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake (2, 3, 5). The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes (1, 6). However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible (7). The extracellular domain shares 79%, 79%, 80% and 67% identity with human, bovine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD.

References
  1. Mian, I.S. (1998) Blood Cells Mol. Dis. 24:83.
  2. Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
  3. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
  4. Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
  5. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
  6. Chang, Q. et al. (2005) Science 310:490.
  7. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
Entrez Gene IDs
152831 (Human); 83379 (Mouse); 289625 (Rat); 102147155 (Cynomolgus Monkey)
Alternate Names
betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; klotho beta-like protein; MGC142213

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Citation for Mouse Klotho  beta Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. TNF-alpha represses beta-Klotho expression and impairs FGF21 action in adipose cells: involvement of JNK1 in the FGF21 pathway.
    Authors: Diaz-Delfin J, Hondares E, Iglesias R, Giralt M, Caelles C, Villarroya F
    Endocrinology, 2012-07-09;153(9):4238-45.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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