Mouse L‑Selectin/CD62L Antibody

  • Specificity
    Detects mouse L‑Selectin/CD62L in Western blots. In Western blots, no cross-reactivity with recombinant human L-Selectin, recombinant mouse (rm) E-Selectin, or rmP-Selectin is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 95226
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse L‑Selectin/CD62L
    Accession # P18337
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
  • Western Blot
    1 µg/mL
    Recombinant Mouse L-Selectin/CD62L Fc Chimera (Catalog # 576-LS)
  • Flow Cytometry
    2.5 µg/106 cells
    Mouse splenocytes
    • Mouse L-Selectin/CD62L Sandwich Immunoassay
  • ELISA Capture
    2-8 µg/mL 
    Mouse L‑Selectin/CD62L Antibody (Catalog # MAB5762)
  • ELISA Detection
    0.5-2.0 µg/mL 
    Mouse L‑Selectin/CD62L Biotinylated Antibody (Catalog # BAM5761)
  • ELISA Standard
    Recombinant Mouse L-Selectin/CD62L Fc Chimera Protein, CF (Catalog # 576-LS)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: L-Selectin/CD62L

L‑Selectin (Leukocyte Selectin; also known as CD62L, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL‑14 antigen, DREG, and lymph node homing receptor), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L‑Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is 90‑100 kDa. Human and mouse L‑Selectin share 76% amino acid sequence homology. L‑Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L‑Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium. This initial interaction involving ICAM-1 and VCAM-1 leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble L‑Selectin are present in the biological fluids of apparently normal individuals. Levels of L‑Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

  • Alternate Names:
    CD62L; hLHRc; LAM1; LECAM1; Leu-8; LNHR; LSEL; LYAM1; Lyam-1; PLNHR; SELL; TQ1; CD62L; CD62L antigen; gp90-MEL; hLHRc; LAM-1; LAM1LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHRTQ1; LSEL; L-selectin; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L
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