Mouse LDL R Antibody

Catalog # Availability Size / Price Qty
MAB2255
MAB2255-SP
Detection of mouse LDL R by Western Blot.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Mouse LDL R Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LDL R in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LDL R or recombinant mouse LRP-6 is observed.
Source
Monoclonal Rat IgG2A Clone # 263123
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LDL R
Ala22-Arg790 (Ala23Val, Cys27Gly)
Accession # Q6GTJ9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of mouse LDL R by Western Blot. View Larger

Detection of mouse LDL R by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse LDL R Monoclonal Antibody (Catalog # MAB2255) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LDL R at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection  of LDL R in RAW 264.7 Mouse Cell Line by Flow Cytometry. View Larger

Detection of LDL R in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse LDL R Monoclonal Antibody (Catalog # MAB2255, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LDL R

The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of scavenger receptors (1, 2, 3, 4). This family contains type I transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities (2, 4). Mouse LDL R is synthesized as a 864 amino acid (aa) precursor that contains a 21 aa signal sequence, a 769 aa extracellular region, a 22 aa transmembrane segment and a 52 aa cytoplasmic tail (5). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats (class A LDL domains) that bind LDL. Cysteines in this region participate in intrachain disulfide bonds. This region is followed by two EGF-like domains and six class B LDL repeats that generate a beta -propeller whose blades each contain a YWTD motif. This area is likely responsible for ligand dissociation (6). Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that shows extensive O-linked glycosylation, generating a native molecular weight for LDL R of 135 kDa (5). Within the 52 aa cytoplasmic region, there is an NPxY motif that links the receptor to clathrin pits and binds to select adaptor proteins (1, 7, 8). The extracellular region of mouse LDL R shares 78% and 87% aa identity with the extracellular region of human and rat LDL R, respectively. LDL R is constitutively expressed and binds apoB of LDL and apoE of VLDL (9). It is responsible for clearing 70% of plasma LDL in liver (9).

 

References
  1. Strickland, D.K. et al. (2002) Trends Endocrinol. Metab. 13:66.
  2. Nykjaer, A. and T.E. Willnow (2002) Trends Cell Biol. 12:273.
  3. Gent, J. and I. Braakman (2004) Cell. Mol. Life Sci. 61:2461.
  4. Bujo, H. and Y. Saito (2006) Arterioscler. Thromb. Vasc. Biol. 26:1246.
  5. Hoffer, M.J. V. et al. (1993) Biochem. Biophys. Res. Commun. 191:880.
  6. Rudenko, G. and J. Deisenhofer (2003) Curr. Opin. Struct. Biol. 13:683.
  7. Trommsdorff, M. et al. (1998) J. Biol. Chem. 273:33556.
  8. Stolt, P.C. and H.H. Bock (2006) Cell. Signal. 18:1560
  9. Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
Long Name
Low Density Lipoprotein Receptor
Entrez Gene IDs
3949 (Human); 16835 (Mouse); 300438 (Rat)
Alternate Names
FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor

Product Datasheets

Citation for Mouse LDL R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. PCSK9 reduces the protein levels of the LDL receptor in mouse brain during development and after ischemic stroke.
    Authors: Rousselet E, Marcinkiewicz J, Kriz J, Zhou A, Hatten ME, Prat A, Seidah NG
    J. Lipid Res., 2011;52(7):1383-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse LDL R Antibody

There are currently no reviews for this product. Be the first to review Mouse LDL R Antibody and earn rewards!

Have you used Mouse LDL R Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review