Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 261639
View all LIGHT/TNFSF14 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse LIGHT
Asp72-Val239
Accession # Q9QYH9

Specificity

Detects mouse LIGHT in direct ELISAs and Western blots. In Western blots, this antibody shows less than 10% cross-reactivity with recombinant mouse (rm) EDA, rmTRAIL, rmTRANCE, rmTWEAK, and rhVEGI but does not cross-react rm4-1BB Ligand, rhAPRIL, rmBAFF, rhEDA-A2, rmFas Ligand, rhGITR Ligand, rmGITR Ligand, rhLymphotoxin alpha

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse LIGHT/TNFSF14 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse LIGHT/TNFSF14 (Catalog # 1794-LT)

Reviewed Applications

Read 1 review rated 5 using MAB1794 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIGHT/TNFSF14

LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) is a member of the TNF superfamily and is designated TNFSF14. The gene for mouse LIGHT encodes a 239 amino acid residue (aa) type II transmembrane glycoprotein that contains a 37 aa N-terminal cytoplasmic domain, a 21 aa transmembrane region, and a 181 aa extracellular domain. A soluble form of mouse LIGHT is generated from the membrane form by proteolytic processing. Similar to other TNF ligand family members, LIGHT is assembled as a homotrimer. Mouse and human LIGHT share 71% aa sequence identity. LIGHT is expressed by activated lymphocytes, natural killer cells, immature dendritic cells, monocytes and granulocytes. Mouse LIGHT binds and signals via two distinct TNF receptor superfamily members, including the herpes virus entry mediator (HVEM/TNFRSF14) and the lymphotoxin beta receptor (LT beta R/TNFRSF3). In humans, LIGHT also binds the soluble human decoy receptor 3 (DcR3/TNFRSF6B). Signaling from LT beta R, which also binds LT alpha beta, induces apoptosis and the production of various cytokines. LIGHT-LT beta R signaling also plays a role in mesenteric lymph node organogenesis, and restoration of secondary lymphoid structure and function. Signaling from HVEM, which also binds LT alpha, co-stimulates T-helper cell type 1 (TH1) immune responses, enhances Cytotoxic T Lymphocytes (CTL)-mediated tumor immunity, and regulates allogeneic T cell activation and allograft rejection. Blockade of LIGHT-HVEM signaling has been shown to prevent graft versus host disease.

References

  1. Granger, S.W. and S. Rickert (2003) Cytokine Growth Factor Rev. 14:289.

Long Name

TNF Ligand Superfamily Member 14

Alternate Names

CD258, TNFSF14

Entrez Gene IDs

8740 (Human); 50930 (Mouse)

Gene Symbol

TNFSF14

UniProt

Q9QYH9

Additional LIGHT/TNFSF14 Products

Product Documents for Mouse LIGHT/TNFSF14 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse LIGHT/TNFSF14 Antibody

For research use only

Citations for Mouse LIGHT/TNFSF14 Antibody

Customer Reviews for Mouse LIGHT/TNFSF14 Antibody (1)

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  • Mouse LIGHT/TNFSF14 Antibody
    Name: Khuynh Nguyen
    Application: Western Blot
    Sample Tested: Mouse LIGHT−/− and bronchoalveolar lavage
    Species: Mouse
    Verified Customer | Posted 03/15/2024
    Mouse LIGHT/TNFSF14 Antibody MAB1794

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