Mouse LIMPII/SR-B2 Lumenal Loop Antibody Summary
Accession # O35114
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of LIMPII/SR-B2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse LIMPII/SR-B2 Affinity-purified Polyclonal Antibody (Catalog # AF1888, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1‑3). SR-B/CD36 family members are type III integral membrane proteins with an N‑ as well as a C-terminal cytoplasmic tail, and a large extracellular (or luminal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is expressed on all tissues and cell types examined so far, including activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII‑thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation and inflammation (1). Overexpression of LIMPII causes an enlargement of early and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5).
- Crombie, R. and R. Silverstein (1998) J. Biol. Chem. 273:4855.
- Febbraio, M. et al. (2001) J. Clin. Invest. 108:785.
- Eskelinen, E-L. et al. (2003) Trends in Cell Biol. 13:137.
- Kuronita, T. et al. (2002) J. Cell Sci. 115:4117.
- Gamp, A-C. et al. (2003) Human Mol. Genet. 12:631.
Citations for Mouse LIMPII/SR-B2 Lumenal Loop Antibody
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Citations: Showing 1 - 3
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Lung immunoglobulin A immunity dysregulation in cystic fibrosis
Authors: AM Collin, M Lecocq, S Noel, B Detry, FM Carlier, F Aboubakar, C Bouzin, T Leal, M Vermeersch, V De Rose, L Regard, C Martin, PR Burgel, D Hoton, S Verleden, A Froidure, C Pilette, S Gohy
Sample Types: BALF
Applications: ELISA Detection
HSL-knockout mouse testis exhibits class B scavenger receptor upregulation and disrupted lipid raft microdomains.
Authors: Casado M, Huerta L, Ortiz A, Perez-Crespo M, Gutierrez-Adan A, Kraemer F, Lasuncion M, Busto R, Martin-Hidalgo A
J Lipid Res, 2012;53(12):2586-97.
Sample Types: Whole Tissue
Identification of a Human SCARB2 Region That Is Important for Enterovirus 71 Binding and Infection.
Authors: Yamayoshi S, Koike S,
J. Virol., 2011;85(10):4937-46.
Sample Types: Cell Lysates
Applications: Western Blot
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