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Mouse Ly-6G/Ly-6C (Gr-1) Alexa Fluor® 594-conjugated Antibody

FAB1037T Datasheet / COA / SDS
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FAB1037T-100UG
R&D Systems Antibodies
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Mouse Ly-6G/Ly-6C (Gr-1) Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects Ly-6G/Ly-6C (Gr-1). Weak cross-reactivity with Ly-6C is observed.
Source
Monoclonal Rat IgG2b Clone # RB6-8C5
Immunogen
The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Mouse splenocytes or peripheral blood cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Ly-6G/Ly-6C (Gr-1)

The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References
  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
  3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
  4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
Long Name
A Myeloid Differentiation Antigen
Entrez Gene IDs
546644 (Mouse)
Alternate Names
C6orf23; G6D; Ly-6C; LY6-D; Ly6G; Ly-6G; Ly-6G/Ly-6C (Gr-1); LY6G6D; lymphocyte antigen 6 family member G6D; MEGT1; NG25

Product Datasheets

Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Rat IgG2B Alexa Fluor® 594-conjugated Isotype Control

IC013T
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Staining Reagents

Flow Cytometry Human Lyse Buffer (10X)

FC002
2 Citations
1 Review
Flow

Flow Cytometry Mouse Lyse Buffer (10X)

FC003
5 Citations
1 Review
Flow

Flow Cytometry Staining Buffer (1X)

FC001
23 Citations
5 Reviews
Flow

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