Mouse Ly-6G/Ly-6C (Gr-1) Antibody

(20 citations)
(3 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects Ly-6G/Ly-6C (Gr-1). Weak cross-reactivity with Ly-6C is observed.
  • Source
    Monoclonal Rat IgG2B Clone # RB6-8C5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    Mouse splenocytes or peripheral blood cells
  • Immunohistochemistry
    Nitta, H. et al. (1997) Cell Vision 4:73.
  • Immunoprecipitation
    Conlan, J.W. and R.J. North (1994) J. Exp. Med. 179:259.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Ly-6G/Ly-6C (Gr-1) in Mouse Spleno­cytes.
Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed mouse splenocytes using Mouse
Ly-6G/Ly-6C (Gr-1) Monoclonal Anti­body (Catalog # MAB1037) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
  Ly-6G/Ly-6C (Gr-1) in Mouse Spleen.
 Ly-6G/Ly-6C (Gr-1) was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1)Monoclonal Antibody (Catalog # MAB1037) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ly-6G/Ly-6C (Gr-1)
The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).
  • References:
    1. Spangrude, G.J. et al. (1988) Science 241:58.
    2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
    3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
    4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
  • Long Name:
    A Myeloid Differentiation Antigen
  • Entrez Gene IDs:
    546644 (Mouse); 546644 (Mouse)
  • Alternate Names:
    Ly-6C; Ly6G; Ly-6G; Ly-6G/Ly-6C (Gr-1)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Monocytic and granulocytic myeloid derived suppressor cells differentially regulate spatiotemporal tumour plasticity during metastatic cascade
    Authors: M Ouzounova, E Lee, R Piranliogl, A El Andalou, R Kolhe, MF Demirci, D Marasco, I Asm, A Chadli, KA Hassan, M Thangaraju, G Zhou, AS Arbab, JK Cowell, H Korkaya
    Nat Commun, 2017;8(0):14979.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Discovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33
    Sci Rep, 2016;6(0):30555.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  3. Rhadinovirus host entry by co-operative infection.
    Authors: Lawler C, Milho R, May J, Stevenson P
    PLoS Pathog, 2015;11(3):e1004761.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  4. C1q Deficiency Promotes Pulmonary Vascular Inflammation and Enhances the Susceptibility of the Lung Endothelium to Injury.
    Authors: Shah D, Romero F, Zhu Y, Duong M, Sun J, Walsh K, Summer R
    J Biol Chem, 2015;290(49):29642-51.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  5. The Src-Family Kinases Hck and Fgr Regulate Early Lipopolysaccharide-Induced Myeloid Cell Recruitment into the Lung and Their Ability To Secrete Chemokines.
    Authors: Mazzi P, Caveggion E, Lapinet-Vera J, Lowell C, Berton G
    J Immunol, 2015;195(5):2383-95.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. Miro1 regulates intercellular mitochondrial transport & enhances mesenchymal stem cell rescue efficacy.
    Authors: Ahmad T, Mukherjee S, Pattnaik B, Kumar M, Singh S, Kumar M, Rehman R, Tiwari B, Jha K, Barhanpurkar A, Wani M, Roy S, Mabalirajan U, Ghosh B, Agrawal A
    EMBO J, 2014;33(9):994-1010.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Transcription factor binding site analysis identifies FOXO transcription factors as regulators of the cutaneous wound healing process.
    Authors: Roupe K, Veerla S, Olson J, Stone E, Sorensen O, Hedrick S, Nizet V
    PLoS ONE, 2014;9(2):e89274.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  8. CCR1 plays a critical role in modulating pain through hematopoietic and non-hematopoietic cells.
    Authors: Lewis, Nuruddee, Muthukumarana, Akalushi, Fogal, Steven E, Corradini, Laura, Stefanopoulos, Dimitria, Adusumalli, Prathima, Pelletier, Josephin, Panzenbeck, Mark, Berg, Karen, Canfield, Melissa, Cook, Brian N, Razavi, Hossein, Kuzmich, Daniel, Anderson, Shawn, Allard, Devan, Harrison, Paul, Grimaldi, Christin, Souza, Donald, Harcken, Christia, Fryer, Ryan M, Modis, Louise K, Brown, Maryanne
    PLoS ONE, 2014;9(8):e105883.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
    Authors: Kowalewska P, Patrick A, Fox-Robichaud A
    PLoS ONE, 2014;9(9):e104537.
    Species: Mouse
    Sample Type: In Vivo
    Application: IHC Fresh
  10. Pseudomonas aeruginosa MucD protease mediates keratitis by inhibiting neutrophil recruitment and promoting bacterial survival.
    Authors: Mochizuki Y, Suzuki T, Oka N, Zhang Y, Hayashi Y, Hayashi N, Gotoh N, Ohashi Y
    Invest Ophthalmol Vis Sci, 2014;55(1):240-6.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  11. Decidual neutrophil infiltration is not required for preterm birth in a mouse model of infection-induced preterm labor.
    Authors: Rinaldi S, Catalano R, Wade J, Rossi A, Norman J
    J Immunol, 2014;192(5):2315-25.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  12. CRTH2 Is A Critical Regulator of Neutrophil Migration and Resistance to Polymicrobial Sepsis.
    Authors: Ishii M, Asano K, Namkoong H, Tasaka S, Mizoguchi K, Asami T, Kamata H, Kimizuka Y, Fujiwara H, Funatsu Y, Kagawa S, Miyata J, Ishii K, Nakamura M, Hirai H, Nagata K, Kunkel SL, Hasegawa N, Betsuyaku T
    J. Immunol., 2012;188(11):5655-64.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  13. Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice.
    Blood, 2012;119(26):6382-93.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  14. The leukotriene B4 receptor, BLT1, is required for the induction of experimental autoimmune encephalomyelitis.
    Authors: Kihara Y, Yokomizo T, Kunita A, Morishita Y, Fukayama M, Ishii S, Shimizu T
    Biochem. Biophys. Res. Commun., 2010;394(3):673-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  15. Uropathogenic Escherichia coli modulates immune responses and its curli fimbriae interact with the antimicrobial peptide LL-37.
    Authors: Kai-Larsen Y, Luthje P, Chromek M, Peters V, Wang X, Holm A, Kadas L, Hedlund KO, Johansson J, Chapman MR, Jacobson SH, Romling U, Agerberth B, Brauner A
    PLoS Pathog., 2010;6(0):e1001010.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  16. TLR4 activation is required for IL-17-induced multiple tissue inflammation and wasting in mice.
    Authors: Tang H, Pang S, Wang M
    J. Immunol., 2010;185(4):2563-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  17. Syndecan-1 shedding facilitates the resolution of neutrophilic inflammation by removing sequestered CXC chemokines.
    Authors: Hayashida K, Parks WC, Park PW
    Blood, 2009;114(14):3033-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  18. Myelomonocytic cell recruitment causes fatal CNS vascular injury during acute viral meningitis.
    Authors: Kim JV, Kang SS, Dustin ML, McGavern DB
    Nature, 2009;457(7226):191-5.
    Species: Mouse
    Sample Type: In Vivo
    Application: Immunodepletion
  19. NOD1 expression in the eye and functional contribution to IL-1beta-dependent ocular inflammation in mice.
    Authors: Rosenzweig HL, Galster KT, Planck SR, Rosenbaum JT
    Invest. Ophthalmol. Vis. Sci., 2009;50(4):1746-53.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  20. Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression.
    Authors: Fujimoto H, Sangai T, Ishii G, Ikehara A, Nagashima T, Miyazaki M, Ochiai A
    Int. J. Cancer, 2009;125(6):1276-84.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Expand to show all 20 Citations
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Average Rating: 5 (Based on 3 reviews)

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We have 3 reviews tested in 1 species: Mouse.
We have 3 reviews tested in 2 applications: Immunohistochemistry, Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Mouse Ly-6G/Ly-6C (Gr-1) Antibody MAB1037
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Excellent
 IHC Mouse Anonymous 12/01/2017
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Immunohistochemistry Mouse Ly-6G/Ly-6C (Gr-1) Antibody MAB1037
Immunohistochemistry: Mouse Ly-6G/Ly-6C (Gr-1) Antibody [MAB1037]

Summary

ApplicationImmunohistochemistry
Sample Testedcolon
SpeciesMouse

Other Experimental Details

Other Experimental DetailsA. Normal mouse colon; B. DSS induced mouse colitis colon.The staining only work at 1:20 dilution instead of the recommended ~1:50 dilution. We tried 4 difference sources and this is the best one.
  Excellent
 Flow Mouse Anonymous 07/17/2017
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Summary

ApplicationFlow Cytometry
Sample TestedGranulocytes
SpeciesMouse
  Excellent
 Flow Mouse Anonymous 05/30/2017
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Summary

ApplicationFlow Cytometry
Sample TestedNeutrophils
SpeciesMouse

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