Mouse Ly-6G/Ly-6C (Gr-1) Antibody

Catalog # Availability Size / Price Qty
MAB1037-SP
MAB1037-100
MAB1037-500
Ly-6G/Ly-6C (Gr-1) in Mouse Spleno­cytes.
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Product Details
Citations (28)
FAQs
Supplemental Products
Reviews (5)

Mouse Ly-6G/Ly-6C (Gr-1) Antibody Summary

Species Reactivity
Mouse
Specificity
Detects Ly-6G/Ly-6C (Gr-1). Weak cross-reactivity with Ly-6C is observed.
Source
Monoclonal Rat IgG2B Clone # RB6-8C5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes or peripheral blood cells
Immunohistochemistry
Nitta, H. et al. (1997) Cell Vision 4:73.
 
Immunoprecipitation
Conlan, J.W. and R.J. North (1994) J. Exp. Med. 179:259.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Ly-6G/Ly-6C (Gr-1) in Mouse Spleno­cytes. View Larger

Ly-6G/Ly-6C (Gr-1) in Mouse Spleno­cytes.
Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed mouse splenocytes using Mouse
Ly-6G/Ly-6C (Gr-1) Monoclonal Anti­body (Catalog # MAB1037) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry   Ly-6G/Ly-6C (Gr-1)  in Mouse Spleen. View Larger

  Ly-6G/Ly-6C (Gr-1) in Mouse Spleen.
 Ly-6G/Ly-6C (Gr-1) was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1)Monoclonal Antibody (Catalog # MAB1037) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ly-6G/Ly-6C (Gr-1)

The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References
  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
  3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
  4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
Long Name
A Myeloid Differentiation Antigen
Entrez Gene IDs
546644 (Mouse); 546644 (Mouse)
Alternate Names
C6orf23; G6D; Ly-6C; LY6-D; Ly6G; Ly-6G; Ly-6G/Ly-6C (Gr-1); LY6G6D; lymphocyte antigen 6 family member G6D; MEGT1; NG25

Product Datasheets

Citations for Mouse Ly-6G/Ly-6C (Gr-1) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis
    Authors: Y Fujita, A Khateb, Y Li, R Tinoco, T Zhang, H Bar-Yoseph, MA Tam, Y Chowers, E Sabo, S Gerassy-Va, E Starosvets, B James, K Brown, SS Shen-Orr, LM Bradley, PA Tessier, ZA Ronai
    Cell Rep, 2018;24(12):3296-3311.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Group 2 Innate Lymphoid Cells Attenuate Inflammatory Arthritis and Protect from Bone Destruction in Mice
    Authors: Y Omata, M Frech, T Primbs, S Lucas, D Andreev, C Scholtysek, K Sarter, M Kindermann, N Yeremenko, DL Baeten, N Andreas, T Kamradt, A Bozec, A Ramming, G Krönke, S Wirtz, G Schett, MM Zaiss
    Cell Rep, 2018;24(1):169-180.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Vesicular nucleotide transporter mediates ATP release and migration in neutrophils
    Authors: Y Harada, Y Kato, T Miyaji, H Omote, Y Moriyama, M Hiasa
    J. Biol. Chem., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. [EXPRESS] Colchicine alleviates acute postoperative pain but delays wound repair in mice: roles of neutrophils and macrophages
    Authors: A Sukeishi, K Isami, H Hiyama, S Imai, K Nagayasu, H Shirakawa, T Nakagawa, S Kaneko
    Mol Pain, 2017;13(0):1744806917743.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Monocytic and granulocytic myeloid derived suppressor cells differentially regulate spatiotemporal tumour plasticity during metastatic cascade
    Authors: M Ouzounova, E Lee, R Piranliogl, A El Andalou, R Kolhe, MF Demirci, D Marasco, I Asm, A Chadli, KA Hassan, M Thangaraju, G Zhou, AS Arbab, JK Cowell, H Korkaya
    Nat Commun, 2017;8(0):14979.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  6. PTPN22 Is a Critical Regulator of Fc? Receptor-Mediated Neutrophil Activation
    Authors: Sonja Vermeren
    J. Immunol, 2016;197(12):4771-4779.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Diabetic Wounds Exhibit Decreased Ym1 and Arginase Expression with Increased Expression of IL-17 and IL-20
    Authors: Laurie P Shornick
    Adv Wound Care (New Rochelle), 2016;5(11):486-494.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice
    Authors: Clara Jana-Lui Busch
    Hepatology, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Discovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33
    Sci Rep, 2016;6(0):30555.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  10. Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation
    Authors: F Aigner
    Am. J. Transplant., 2016;16(3):808-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Rhadinovirus host entry by co-operative infection.
    Authors: Lawler C, Milho R, May J, Stevenson P
    PLoS Pathog, 2015;11(3):e1004761.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  12. C1q Deficiency Promotes Pulmonary Vascular Inflammation and Enhances the Susceptibility of the Lung Endothelium to Injury.
    Authors: Shah D, Romero F, Zhu Y, Duong M, Sun J, Walsh K, Summer R
    J Biol Chem, 2015;290(49):29642-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  13. The Src-Family Kinases Hck and Fgr Regulate Early Lipopolysaccharide-Induced Myeloid Cell Recruitment into the Lung and Their Ability To Secrete Chemokines.
    Authors: Mazzi P, Caveggion E, Lapinet-Vera J, Lowell C, Berton G
    J Immunol, 2015;195(5):2383-95.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  14. Transcription factor binding site analysis identifies FOXO transcription factors as regulators of the cutaneous wound healing process.
    Authors: Roupe K, Veerla S, Olson J, Stone E, Sorensen O, Hedrick S, Nizet V
    PLoS ONE, 2014;9(2):e89274.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. CCR1 plays a critical role in modulating pain through hematopoietic and non-hematopoietic cells.
    Authors: Lewis, Nuruddee, Muthukumarana, Akalushi, Fogal, Steven E, Corradini, Laura, Stefanopoulos, Dimitria, Adusumalli, Prathima, Pelletier, Josephin, Panzenbeck, Mark, Berg, Karen, Canfield, Melissa, Cook, Brian N, Razavi, Hossein, Kuzmich, Daniel, Anderson, Shawn, Allard, Devan, Harrison, Paul, Grimaldi, Christin, Souza, Donald, Harcken, Christia, Fryer, Ryan M, Modis, Louise K, Brown, Maryanne
    PLoS ONE, 2014;9(8):e105883.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  16. Pseudomonas aeruginosa MucD protease mediates keratitis by inhibiting neutrophil recruitment and promoting bacterial survival.
    Authors: Mochizuki Y, Suzuki T, Oka N, Zhang Y, Hayashi Y, Hayashi N, Gotoh N, Ohashi Y
    Invest Ophthalmol Vis Sci, 2014;55(1):240-6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  17. Miro1 regulates intercellular mitochondrial transport & enhances mesenchymal stem cell rescue efficacy.
    Authors: Ahmad T, Mukherjee S, Pattnaik B, Kumar M, Singh S, Kumar M, Rehman R, Tiwari B, Jha K, Barhanpurkar A, Wani M, Roy S, Mabalirajan U, Ghosh B, Agrawal A
    EMBO J, 2014;33(9):994-1010.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  18. Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
    Authors: Kowalewska P, Patrick A, Fox-Robichaud A
    PLoS ONE, 2014;9(9):e104537.
    Species: Mouse
    Sample Types: In Vivo
    Applications: IHC Fresh
  19. Decidual neutrophil infiltration is not required for preterm birth in a mouse model of infection-induced preterm labor.
    Authors: Rinaldi S, Catalano R, Wade J, Rossi A, Norman J
    J Immunol, 2014;192(5):2315-25.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  20. Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice.
    Blood, 2012;119(26):6382-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  21. CRTH2 Is A Critical Regulator of Neutrophil Migration and Resistance to Polymicrobial Sepsis.
    Authors: Ishii M, Asano K, Namkoong H, Tasaka S, Mizoguchi K, Asami T, Kamata H, Kimizuka Y, Fujiwara H, Funatsu Y, Kagawa S, Miyata J, Ishii K, Nakamura M, Hirai H, Nagata K, Kunkel SL, Hasegawa N, Betsuyaku T
    J. Immunol., 2012;188(11):5655-64.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  22. Uropathogenic Escherichia coli modulates immune responses and its curli fimbriae interact with the antimicrobial peptide LL-37.
    Authors: Kai-Larsen Y, Luthje P, Chromek M, Peters V, Wang X, Holm A, Kadas L, Hedlund KO, Johansson J, Chapman MR, Jacobson SH, Romling U, Agerberth B, Brauner A
    PLoS Pathog., 2010;6(0):e1001010.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  23. TLR4 activation is required for IL-17-induced multiple tissue inflammation and wasting in mice.
    Authors: Tang H, Pang S, Wang M
    J. Immunol., 2010;185(4):2563-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  24. The leukotriene B4 receptor, BLT1, is required for the induction of experimental autoimmune encephalomyelitis.
    Authors: Kihara Y, Yokomizo T, Kunita A, Morishita Y, Fukayama M, Ishii S, Shimizu T
    Biochem. Biophys. Res. Commun., 2010;394(3):673-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. NOD1 expression in the eye and functional contribution to IL-1beta-dependent ocular inflammation in mice.
    Authors: Rosenzweig HL, Galster KT, Planck SR, Rosenbaum JT
    Invest. Ophthalmol. Vis. Sci., 2009;50(4):1746-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  26. Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression.
    Authors: Fujimoto H, Sangai T, Ishii G, Ikehara A, Nagashima T, Miyazaki M, Ochiai A
    Int. J. Cancer, 2009;125(6):1276-84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  27. Syndecan-1 shedding facilitates the resolution of neutrophilic inflammation by removing sequestered CXC chemokines.
    Authors: Hayashida K, Parks WC, Park PW
    Blood, 2009;114(14):3033-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  28. Myelomonocytic cell recruitment causes fatal CNS vascular injury during acute viral meningitis.
    Authors: Kim JV, Kang SS, Dustin ML, McGavern DB
    Nature, 2009;457(7226):191-5.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Immunodepletion

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Reviews for Mouse Ly-6G/Ly-6C (Gr-1) Antibody

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Mouse Ly-6G/Ly-6C (Gr-1) Antibody
By Anonymous on 04/18/2018
Application: ICC/IF Sample Tested: Lung cancer tissue Species: Mouse

Mouse Ly-6G/Ly-6C (Gr-1) Antibody
By Anonymous on 04/11/2018
Application: Flow Sample Tested: Pancreatic cancer cells Species: pancreatic cancer cell line,Mouse

Mouse Ly-6G/Ly-6C (Gr-1) Antibody
By Anonymous on 12/01/2017
Application: IHC Sample Tested: colon Species: Mouse

A. Normal mouse colon; B. DSS induced mouse colitis colon.
The staining only work at 1:20 dilution instead of the recommended ~1:50 dilution.

We tried 4 difference sources and this is the best one.


Mouse Ly-6G/Ly-6C (Gr-1) Antibody
By Anonymous on 07/17/2017
Application: Flow Sample Tested: Granulocytes Species: Mouse

Mouse Ly-6G/Ly-6C (Gr-1) Antibody
By Anonymous on 05/30/2017
Application: Flow Sample Tested: Neutrophils Species: Mouse