Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Gr-1/Ly-6G. Weak cross-reactivity with Ly-6C is observed.
  • Source
    Monoclonal Rat IgG2B Clone # RB6-8C5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 405
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Gr‑1/Ly‑6G in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Integrin  alpha M/CD11b APC‑conjugated Monoclonal Antibody (Catalog # FAB1124A) and either (A) Rat Anti-Mouse Gr‑1/Ly‑6G Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB1037V) or (B) Rat IgG2B Alexa Fluor 405 Isotype Control (Catalog # IC013V). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Ly-6G/Ly-6C (Gr-1)
The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).
  • References:
    1. Spangrude, G.J. et al. (1988) Science 241:58.
    2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
    3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
    4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
  • Long Name:
    A Myeloid Differentiation Antigen
  • Entrez Gene IDs:
    546644 (Mouse); 546644 (Mouse)
  • Alternate Names:
    Ly-6C; Ly6G; Ly-6G; Ly-6G/Ly-6C (Gr-1)
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 405-conjugated Isotype Control

Ctrl IC013V
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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