Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1037V-025
Detection of Gr‑1/Ly‑6G in Mouse Bone Marrow Cells by Flow Cytometry.
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Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Gr-1/Ly-6G. Weak cross-reactivity with Ly-6C is observed.
Source
Monoclonal Rat IgG2B Clone # RB6-8C5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Gr‑1/Ly‑6G in Mouse Bone Marrow Cells by Flow Cytometry. View Larger

Detection of Gr‑1/Ly‑6G in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Integrin  alpha M/CD11b APC‑conjugated Monoclonal Antibody (Catalog # FAB1124A) and either (A) Rat Anti-Mouse Gr‑1/Ly‑6G Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB1037V) or (B) Rat IgG2B Alexa Fluor 405 Isotype Control (Catalog # IC013V). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Ly-6G/Ly-6C (Gr-1)

The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References
  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
  3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
  4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
Long Name
A Myeloid Differentiation Antigen
Entrez Gene IDs
546644 (Mouse); 546644 (Mouse)
Alternate Names
C6orf23; G6D; Ly-6C; LY6-D; Ly6G; Ly-6G; Ly-6G/Ly-6C (Gr-1); LY6G6D; lymphocyte antigen 6 family member G6D; MEGT1; NG25

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