Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin

Label

Biotin

Antibody Source

Monoclonal Rat IgG2B Clone # RB6-8C5
View all Ly-6G/Ly-6C (Gr-1) Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is Ly-6G (Gr-1, Gr1).

Specificity

Detects mouse Ly-6G/Ly-6C (Gr-1). Weak cross-reactivity with Ly-6C is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

Ly-6G/Ly-6C (Gr-1) antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr).

Ly-6G/Ly-6C (Gr-1) in Mouse Spleen.

Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Monoclonal Antibody (Catalog # BAM1037) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes

Immunohistochemistry

Nitta, H. et al. (1997) Cell Vision 4:73.

Immunoprecipitation

Conlan, J.W. and R.J. North (1994) J. Exp. Med. 179:259.

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ly-6G/Ly-6C (Gr-1)

Gr-1, also called Ly-6G, is a myeloid differentiation antigen that is a member of the Ly-6 family. The RB6‑8C5 antibody recognizes Gr-1/Ly-6G, and also reacts weakly with Ly-6C-transfected EL4 cells (1, 2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References

  1. Spangrude, G.J. et al. (1988) Science 241:58
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399
  3. Lewinsohn, D.M. et al. (1987) J. Immunol. 147:22
  4. Lagasse, E. and I. L. Weissman (1996) J. Immunol. Methods 197:139

Long Name

A Myeloid Differentiation Antigen

Alternate Names

Ly-6C, Ly-6G, Ly6G

Entrez Gene IDs

546644 (Mouse)

Gene Symbol

LY6G

Additional Ly-6G/Ly-6C (Gr-1) Products

Product Documents for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

For research use only

Citations for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

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Protocols

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FAQs

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