Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Ly-6G/Ly-6C (Gr-1). Weak cross-reactivity with Ly-6C is observed.
  • Source
    Monoclonal Rat IgG2B Clone # RB6-8C5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    Mouse splenocytes
  • Immunohistochemistry
    Nitta, H. et al. (1997) Cell Vision 4:73.
  • Immunoprecipitation
    Conlan, J.W. and R.J. North (1994) J. Exp. Med. 179:259.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Ly-6G/Ly-6C (Gr-1) in Mouse Spleen. Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Monoclonal Antibody (Catalog # BAM1037) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ly-6G/Ly-6C (Gr-1)
Gr-1, also called Ly-6G, is a myeloid differentiation antigen that is a member of the Ly-6 family. The RB6‑8C5 antibody recognizes Gr-1/Ly-6G, and also reacts weakly with Ly-6C-transfected EL4 cells (1, 2). In the periphery, this antibody specifically recognized granulocytes (3, 4).
  • References:
    1. Spangrude, G.J. et al. (1988) Science 241:58
    2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399
    3. Lewinsohn, D.M. et al. (1987) J. Immunol. 147:22
    4. Lagasse, E. and I. L. Weissman (1996) J. Immunol. Methods 197:139
  • Long Name:
    A Myeloid Differentiation Antigen
  • Entrez Gene IDs:
    546644 (Mouse)
  • Alternate Names:
    Gr-1; Ly-6C; Ly6G; Ly-6G; Ly-6G/Ly-6C (Gr-1)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Oral chymase inhibitor SUN13834 ameliorates skin inflammation as well as pruritus in mouse model for atopic dermatitis.
    Authors: Terakawa M, Fujieda Y, Tomimori Y, Muto T, Tanaka T, Maruoka H, Nagahira K, Ogata A, Nakatsuka T, Fukuda Y
    Eur. J. Pharmacol., 2008;601(1):186-91.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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