Ly-6G/Ly-6C (Gr-1) in Mouse Spleen. Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Monoclonal Antibody (Catalog # BAM1037) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ly-6G/Ly-6C (Gr-1)
Gr-1, also called Ly-6G, is a myeloid differentiation antigen that is a member of the Ly-6 family. The
RB6‑8C5 antibody recognizes Gr-1/Ly-6G, and also reacts weakly with Ly-6C-transfected EL4 cells
(1, 2). In the periphery, this antibody specifically recognized granulocytes (3, 4).
Spangrude, G.J. et al. (1988) Science 241:58
Fleming, T.J. et al. (1993) J. Immunol. 151:2399
Lewinsohn, D.M. et al. (1987) J. Immunol. 147:22
Lagasse, E. and I. L. Weissman (1996) J. Immunol. Methods 197:139
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but also provides information about sample types, species, and experimental conditions.
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