Home / Ly-6G/Ly-6C (Gr-1) / Mouse Ly-6G/Ly-6C (Gr-1) MAb (Clone RB6-8C5)

Mouse Ly-6G/Ly-6C (Gr-1) MAb (Clone RB6-8C5)

Catalog #: MLDP6
1 Citation Datasheet / COA / SDS
Newer Version Available: MAB1037
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MLDP6 has been discontinued and is replaced by MAB1037.

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Mouse Ly-6G/Ly-6C (Gr-1) MAb (Clone RB6-8C5) Summary

Species Reactivity
Mouse
Specificity
This antibody detects the myeloid differentiation antigen (Gr-1).1 Gr-1 has been shown to be a member of the Ly-6 family, Ly-6G.2 The RB6-8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells.2 In the periphery, this antibody specifically recognized granulocytes.3, 4
Source
Monoclonal Rat IgG2b Clone # RB6-8C5
Label
Unconjugated

Applications

Recommended Concentration
Sample
Cell depletion
This antibody has been used in combination with antibodies to other lineage markers to deplete the cells of major hematopoietic lineages. Each vial contains sufficient antibody to deplete Gr-1+ cells from 1 x 109 bone marrow cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Cell Depletion View Larger

Cell Depletion View Larger

Cell Depletion View Larger

Figure 1:Lineage positive bone marrow cells from C57/Bl6 mice were depleted using monoclonal anti-mouse CD3, CD4, CD5, CD8 alpha, CD11b/MAC-1 alpha, B220, Gr-1 (Ly-6G) and TER-119 erythroid antigen antibodies and stained with goat anti-rat IgG PE-conjugated. Flow cytometric analysis was performed A) Before (dotted histogram) and after (solid histogram) the depletion antibody cocktail was added; B) Before (dotted histogram) and after (solid histogram) magnetic depletion using Dynabeads; and C) Expression of CD117 (SCF R/c-kit) (R&D Systems, Catalog # MAB1356; dotted histogram) in bone marrow progenitor cells after enrichment using the mouse lineage panel and bead depletion.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Ly-6G/Ly-6C (Gr-1)

The mouse Gr-1 antigen is a GPI-linked myeloid differentiation marker also known as Ly-6G. Its cellular expression is restricted to monocytes within bone and on neutrophils in peripheral organs.

References
  1. Current Protocols in Immunology, Isolation of Murine Macrophages, copyright 1994, Coligan, J.E. et al. eds., John Wiley & Sons, Inc., Volume 3, Supplement 11, 14.1.4.
Long Name
A Myeloid Differentiation Antigen
Entrez Gene IDs
546644 (Mouse)
Alternate Names
Gr1; Gr-1; Ly-6C; Ly6G; Ly-6G; ly-6G.1; Ly-6G/Ly-6C (Gr-1); lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6G

Product Datasheets

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Product Datasheet
COA
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Product Specific Notices

Dynabeads® is a registered trademark of Dynal Biotech, Lake Success, NY.

Citation for Mouse Ly-6G/Ly-6C (Gr-1) MAb (Clone RB6-8C5)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Gr-1high polymorphonuclear leukocytes and NK cells act via IL-15 to clear intracellular Haemophilus influenzae in experimental murine peritonitis and pneumonia.
    Authors: Miyazaki S, Ishikawa F, Shimizu K, Ubagai T, Edelstein PH, Yamaguchi K
    J. Immunol., 2007-10-15;179(8):5407-14.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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