Mouse Lymphotoxin-alpha /TNF-beta Biotinylated Antibody

Catalog # Availability Size / Price Qty
Product Details
Supplemental Products

Mouse Lymphotoxin-alpha /TNF-beta Biotinylated Antibody Summary

Species Reactivity
Detects mouse Lymphotoxin‑ alpha /TNF‑ beta in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human Lymphotoxin‑ alpha /TNF‑ beta is observed (non-reducing conditions).
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse Lymphotoxin‑ alpha /TNF‑ beta
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Lymphotoxin‑ alpha /TNF‑ beta
5-15 µg/mL
Perfusion fixed frozen sections of mouse thymus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin-alpha/TNF-beta

Tumor necrosis factor-beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), is a secreted homotrimeric glycoprotein belonging to the TNF superfamily and is designated TNFSF1B. It is produced by NK, T, and B cells. TNF-beta was originally identified as protein that kills tumor cells in cell culture supernatants of a lymphoblastoid cell line. The TNF-beta subunit also associates with the type II transmembrane TNF superfamily protein lymphotoxin beta (LT beta ) to generate two types of heterotrimers designated as LT alpha 1 beta 2 (a single TNF-beta chain non-covalently associated with two chains of LT beta ), and LT alpha 2 beta 1 (1, 2). TNF-alpha, TNF-beta, and LT beta form a subfamily of the TNF related ligands. Their genes are genetically linked within a compact cluster inside the major histocompatibility complex locus (2, 3). The soluble TNF-beta binds and signals through TNF R1 and TNF R2. In contrast, the membrane-bound LT alpha 1 beta 2 interacts specifically with the LT beta  receptor (LT beta R), which does not bind TNF-beta or TNF-alpha. Both TNFR1 and TNFR2 bind LT alpha 2 beta 1, which is recognized weakly by LT beta  R (4, 5). TNF R1 and 2 express very broadly, while expression of LT beta  R is restricted to stromal cells of lymphoid tissues. Herpesvirus entry mediator binds TNF-beta in vitro (6). The physiological importance of such interaction, if it occurs in vivo, is unclear. Distinct functions attributed to TNF-beta from transgenic knock-out mice include, loss of lymph node development, change in splenic architecture, impaired germinal center formation, and susceptibility to pulmonary tuberculosis (7, 8). TNF-beta also has overlapping physiological functions with LT beta and TNF-alpha in lymphoid organogenesis (7). Mouse and human TNF-beta share approximately 74% homology in their amino acid sequence.

  1. Aggarwal, B.B. (2003) Nature Rev. Immunol. 3:745.
  2. Browning, J.L. et al. (1993) Cell 72:846.
  3. Browning, J.L. et al. (1995) J. Immunol. 154:33.
  4. Pokholok, D.K. et al. (1995) Proc. Natl. Acad. Sci. USA 92:674.
  5. Aggarwal, B.B. et al. (1985) Nature 318:665.
  6. Crowe, P.D. et al. (1994) Science 264:707.
  7. Mauri, D.N. et al. (1998) Immunity 8:21.
  8. Tumanov, A.V. et al. (2003) Cytokine & Growth Factor Rev. 14:275.
  9. Roach, D.R. et al. (2001) J. Exp. Med. 193:239.
Long Name
Tumor Necrosis Factor beta
Entrez Gene IDs
4049 (Human); 16992 (Mouse)
Alternate Names
LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Lymphotoxin-alpha /TNF-beta Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse Lymphotoxin-alpha /TNF-beta Biotinylated Antibody and earn rewards!

Have you used Mouse Lymphotoxin-alpha /TNF-beta Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review