Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Mouse IL-4 is synthesized with a 24 aa signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and
-21). The type II receptor on nonhematopoietic cells consists of IL‑4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL‑13 mediated signals. IL-4 is primarily expressed by Th2‑biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11 ‑ 14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).
Recombinant Mouse IL-4 Protein
R&D Systems | Catalog # 404-ML
Key Product Details
- R&D Systems E. coli-derived Recombinant Mouse IL-4 Protein (404-ML)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
His23-Ser140, with an N-terminal Met
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
The ED50 for this effect is 0.300‑3.00 ng/mL.
Reviewed Applications
Read 21 reviews rated 4.8 using 404-ML in the following applications:
Scientific Data Images for Recombinant Mouse IL-4 Protein
Recombinant Mouse IL‑4 Protein SEC-MALS.
Recombinant Mouse IL-4 (Catalog # 404-ML) has a molecular weight (MW) of 13.2 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Recombinant Mouse IL-4 Protein Bioactivity
Recombinant Mouse IL-4 (Catalog # 404-ML) stimulates cell proliferation of the HT‑2 mouse T cell line. The ED50 for this effect is 0.300-3.00 ng/mL.
Recombinant Mouse IL-4 Protein SDS-PAGE
1 μg/lane of Recombinant Mouse IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 12 kDa.Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 404-ML
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 404-ML/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: IL-4
References
- Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
- Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
- Lee, F. et al. (1986) Proc. Natl. Acad. Sci. 83:2061.
- Noma, Y. et al. (1986) Nature 319:640.
- Redfield, C. et al. (1991) Biochemistry 30:11029.
- Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
- Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
- Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
- Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
- Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
- Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
- Corthay, A. (2006) Scand. J. Immunol. 64:93.
- Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
- Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
- Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional IL-4 Products
Product Documents for Recombinant Mouse IL-4 Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse IL-4 Protein
For research use only
Related Research Areas
Citations for Recombinant Mouse IL-4 Protein
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Customer Reviews for Recombinant Mouse IL-4 Protein (21)
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Customer Images
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 07/11/2025
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Application: Cell ProliferationVerified Customer | Posted 11/12/2024
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 04/05/2024Western blot of in vitro-differentiated mouse basophils after indicated time points upon cytokine treatment with IL-4 (20 ng/ml)
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Application: Cell migration/motilityVerified Customer | Posted 07/25/2023
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Application: Immunoassay StandardVerified Customer | Posted 11/14/2022Used as a standard in our mouse ELISA.
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Application: Enzymatic activity in vitroVerified Customer | Posted 09/29/2021
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Application: Cell ProliferationVerified Customer | Posted 05/20/2021
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 04/13/2021
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Application: Cell ProliferationVerified Customer | Posted 02/26/2021
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 01/27/2021
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Application: Cell ProliferationVerified Customer | Posted 01/05/2021
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Application: Cell ProliferationVerified Customer | Posted 01/05/2021
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Application: Cell ProliferationVerified Customer | Posted 11/09/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 10/23/2020concentration :5ng/ml Time: 24 h
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Application: Immune-cell polarization.Verified Customer | Posted 10/06/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 10/16/2019
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Application: Cell ProliferationVerified Customer | Posted 07/13/2018
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 04/02/2018
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 02/07/2018
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 07/21/2016We used recombinant mouse IL-4 to polarize bonemarrow-derived macrophages to an "M2" phenotype in vitro. We first generated macrophages from whole bone marrow using MCSF-rich media for 7 days. Then we added IL-4 at 20 ng/ml for 48 hours. Using IL-4R wild-type or knock-out bone marrow, we demonstrated using flow cytometry for an M2 reporter (Arginase1-YFP) that the IL-4 acts through the IL-4 receptor to polarize bone marrow derived macrophages towards the M2 phenotype.
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 01/21/2016
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FAQs for Recombinant Mouse IL-4 Protein
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Q: Has recombinant mouse IL-4, Catalog # 404-ML, been tested for activity on human cells?
A: We have not evaluated the bioactivity of Recombinant Mouse IL-4 Protein (Catalog # 404-ML) with human cells. However, we recommend reviewing our list of publications under the Citations tab on the product web page to find reported use of this product with human cells. We do offer a Recombinant Human IL-4 Protein (Catalog # 204-IL), which is suitable for use with human cells.
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Q: What concentration of recombinant mouse IL-4, Catalog # 404-ML, should I use for my cell culture experiment?
A: We evaluate the bioactivity of Recombinant Mouse IL-4 Protein (Catalog # 404-ML) in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.5-1.5 ng/mL. This ED50 range can provide a starting point for your experiment, but we would recommend end-users perform a dose-response to determine the best concentration for their assay system. We also recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.
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Q: Has recombinant mouse IL-4, Catalog # 404-ML, been tested for activity on human cells?
A: We have not evaluated the bioactivity of Recombinant Mouse IL-4 Protein (Catalog # 404-ML) with human cells. However, we recommend reviewing our list of publications under the Citations tab on the product web page to find reported use of this product with human cells. We do offer a Recombinant Human IL-4 Protein (Catalog # 204-IL), which is suitable for use with human cells.
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Q: What concentration of recombinant mouse IL-4, Catalog # 404-ML, should I use for my cell culture experiment?
A: We evaluate the bioactivity of Recombinant Mouse IL-4 Protein (Catalog # 404-ML) in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.5-1.5 ng/mL. This ED50 range can provide a starting point for your experiment, but we would recommend end-users perform a dose-response to determine the best concentration for their assay system. We also recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.
Associated Pathways
IL-4 Signaling Pathways
IL-4 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-9 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
