Mouse Mast Cell Protease-1/Mcpt1 Antibody

Catalog #: MAB5146 Datasheet / COA / SDS

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MAB5146
MAB5146-SP

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Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Western Blot.

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Mouse Mast Cell Protease-1/Mcpt1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Mast Cell Protease‑1/Mcpt1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) MCP-6, -7, or ‑11 is observed.

Source

Monoclonal Rat IgG_2B Clone # 285008

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑1/Mcpt1
Ile21-Lys246
Accession # P11034

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

50 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat Mast Cell Protease-1/Mcpt1 Monoclonal Antibody (Catalog # MAB5146) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Mast Cell Protease-1/Mcpt1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Simple Western^TM. Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for Mast Cell Protease-1/Mcpt1 at approximately 34 kDa (as indicated) using 50 µg/mL of Rat Anti-Mouse/Rat Mast Cell Protease-1/Mcpt1 Monoclonal Antibody (Catalog # MAB5146) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mast Cell Protease-1/Mcpt1

Mast cell protease-1 (MCPT-1), also known as beta -chymase, is a member of the Chymase family of chymotrypsin-like serine proteases (1). mMCPT-1 is a mast cell protease predominantly expressed in intestinal mucosal mast cells where it promotes mucosal permeability in intestinal allergic hypersensitivity reactions (2). Its activation is completed by the removal of a two residue N-terminal propeptide by a dipeptidyl peptidase (Cathepsin C) (3). Like human alpha -Chymase, MCPT-1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure (4). Studies have shown that specific chymase inhibitors are able to diminish the development of abdominal aortic aneurysm and reduce the adhesion formation after cardiac surgery in hamsters (5, 6). Therefore, the development of specific inhibitors of chymase activity has been a pharmacologic strategy to develop therapeutic agents.

References

Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.), p. 1531 Academic Press, San Diego.
Wastling, J.M. et al. (1998) Am. J. Pathol. 153:491.
Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
Saito, K. et al. (2003) Biochem. Biophys. Res. Commun. 302:773.
Tsunemi, K. et al. (2004) J. Pharmacol. Exp. Ther. 309:879.
Soga, Y. et al. (2004) J. Thorac. Cardiovasc. Surg. 127:72.