Metadherin (MTDH; also Lyric and Astrocyte-elevated gene-1/AEG1) is a unique molecule originally discovered to be upregulated in astrocytes following HIV infection. Although its predicted MW is 64 kDa, it runs anomalously at approximately 80 kDa in SDS-Page. It is widely expressed, and appears to be a component of the ER, nucleolus and inner nuclear membrane. Metadherin is reported to promote Akt/PI3 kinase activity, and suppress apoptosis-associated FOXO3A transcription. Mouse Metadherin is a type III (i.e.- a type I with no signal sequence) transmembrane protein 579 amino acids (aa) in length. It contains a luminal N-terminus (aa 1-48) with a 510 aa cytoplasmic C-terminus. There are no identifiable structural motifs, although two poly-Lys segments and two utilized Ser phosphorylation sites exist. Multiple bands at 80 kDa, 75 kDa, 50-55 kDa and 37 kDa are seen for Metadherin on SDS-Page. They may reflect the presence of at least two potential isoform variants that show 1) a deletion of aa 422-457, and 2) an Ile substitution for aa 190-579. Over aa 167-297, mouse Metadherin shares 98% and 97% aa sequence identity with rat and human Metadherin, respectively.
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 754523
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Product Specifications
Immunogen
E. coli-derived recombinant mouse Metadherin
Lys167-Ser297
Accession # Q80WJ7
Lys167-Ser297
Accession # Q80WJ7
Specificity
Detects mouse Metadherin in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Metadherin Antibody
Detection of Mouse Metadherin by Western Blot.
Western blot shows lysates of 4T1 mouse breast cancer cell line and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Metadherin Monoclonal Antibody (Catalog # MAB7180) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Metadherin at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse Metadherin Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: 4T1 mouse breast cancer cell line and P19 mouse embryonal carcinoma cell line
Sample: 4T1 mouse breast cancer cell line and P19 mouse embryonal carcinoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB7180 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Metadherin
Alternate Names
3D3, AEG1, LYRIC, MTDH
Gene Symbol
MTDH
UniProt
Additional Metadherin Products
Product Documents for Mouse Metadherin Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Metadherin Antibody
For research use only
Related Research Areas
Citations for Mouse Metadherin Antibody
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Application: Western BlotSample Tested: 4T1 mouse breast cancer cell lineSpecies: MouseVerified Customer | Posted 02/16/2020
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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