Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 347505
View all Meteorin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Meteorin isoform 1
Gly22-Asp291
Accession # Q8C1Q4

Specificity

Detects mouse Meteorin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Meteorin Antibody

Meteorin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

Meteorin in Rat Cortical Stem Cells.

Meteorin was detected in immersion fixed 7 day differentiated rat cortical stem cells using 10 µg/mL Rat Anti-Mouse Meteorin Monoclonal Antibody (Catalog # MAB3475) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse Meteorin Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed 7 day differentiated rat cortical stem cells

Western Blot

1 µg/mL
Sample: Recombinant Mouse Meteorin (Catalog # 3475-MN)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Meteorin

Meteorin, named after meteors because this protein can transform glial cells into cells with elongated tails, is a 30 kDa member of the Meteorin family (1). The secreted protein is also known as glial cell differentiation factor and hypoxia/reoxygenation regulatory factor. Mouse Meteorin is synthesized as a 291 amino acid (aa) precursor with a 21 aa signal sequence and a 270 aa mature chain. Alternative splicing produces two isoforms. Isoform 2 is missing residues 1‑164 found in isomer 1, and has a two aa substitution for aa 165‑166. Mouse Meteorin shares 81% aa identity with human Meteorin (2). Meteorin is expressed in the central nervous system both during development and in adult mice (2). During development, Meteorin mRNA appears both in the central and peripheral nervous systems with the most prominent levels in neural progenitors, glial progenitors, and cells of the astrocyte lineage. In the adult mouse, Meteorin is detected only in the brain (2). Within the brain, the most prominent expression is found in the cerebellum where it is expressed by glial cells interspersed between Purkinje neurons (2). Meteorin is also detectable in several discrete neuronal populations, such as the superior colliculus, the ocular motor nucleus, the raphe and pontine nuclei, and various thalamic nuclei (2). Functionally, Meteorin plays important roles in both glial cell differentiation and axonal network formation during neurogenesis (1). In addition, when Meteorin is expressed and secreted by perivascular astrocytes, it upregulates thrombospondin-1/-2 to attenuate angiogenesis in the surrounding endothelial cells and to promote vascular maturation (3).

References

  1. Nishino, J. et al. (2004) EMBO J. 23:1998.
  2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104.
  3. Park, J.A. et al. (2008) Glia 56:247.

Alternate Names

METRN

Entrez Gene IDs

79006 (Human); 70083 (Mouse); 287151 (Rat)

Gene Symbol

METRN

UniProt

Q8C1Q4 (Mouse)

Additional Meteorin Products

Product Documents for Mouse Meteorin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Meteorin Antibody

For research use only

Related Research Areas

Glial Lineage Markers

Citations for Mouse Meteorin Antibody

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Protocols

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