Mouse Meteorin Antibody

Catalog # Availability Size / Price Qty
MAB3475
MAB3475-SP
Meteorin in Rat Cortical Stem Cells.
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Product Details
Citations (1)
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Mouse Meteorin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Meteorin in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2B Clone # 347505
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Meteorin isoform 1
Gly22-Asp291
Accession # Q8C1Q4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Meteorin (Catalog # 3475-MN)
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Meteorin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Meteorin in Rat Cortical Stem Cells. Meteorin was detected in immersion fixed 7 day differentiated rat cortical stem cells using 10 µg/mL Rat Anti-Mouse Meteorin Monoclonal Antibody (Catalog # MAB3475) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Meteorin

Meteorin, named after meteors because this protein can transform glial cells into cells with elongated tails, is a 30 kDa member of the Meteorin family (1). The secreted protein is also known as glial cell differentiation factor and hypoxia/reoxygenation regulatory factor. Mouse Meteorin is synthesized as a 291 amino acid (aa) precursor with a 21 aa signal sequence and a 270 aa mature chain. Alternative splicing produces two isoforms. Isoform 2 is missing residues 1‑164 found in isomer 1, and has a two aa substitution for aa 165‑166. Mouse Meteorin shares 81% aa identity with human Meteorin (2). Meteorin is expressed in the central nervous system both during development and in adult mice (2). During development, Meteorin mRNA appears both in the central and peripheral nervous systems with the most prominent levels in neural progenitors, glial progenitors, and cells of the astrocyte lineage. In the adult mouse, Meteorin is detected only in the brain (2). Within the brain, the most prominent expression is found in the cerebellum where it is expressed by glial cells interspersed between Purkinje neurons (2). Meteorin is also detectable in several discrete neuronal populations, such as the superior colliculus, the ocular motor nucleus, the raphe and pontine nuclei, and various thalamic nuclei (2). Functionally, Meteorin plays important roles in both glial cell differentiation and axonal network formation during neurogenesis (1). In addition, when Meteorin is expressed and secreted by perivascular astrocytes, it upregulates thrombospondin-1/-2 to attenuate angiogenesis in the surrounding endothelial cells and to promote vascular maturation (3).

References
  1. Nishino, J. et al. (2004) EMBO J. 23:1998.
  2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104.
  3. Park, J.A. et al. (2008) Glia 56:247.
Entrez Gene IDs
79006 (Human); 70083 (Mouse); 287151 (Rat)
Alternate Names
C16orf23; c380A1.2; Meteorin; METRN

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Citation for Mouse Meteorin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Meteorin links the bone marrow hypoxic state to hematopoietic stem/progenitor cell mobilization
    Authors: YW Dai, JK Ma, R Jiang, XL Zhan, SY Chen, LL Feng, Q Zhang, TB Liang, K Lv, GJ Yang, JF Lu, J Chen, XJ Lu
    Cell Reports, 2022-09-20;40(12):111361.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP, Western Blot

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