Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse

Applications

Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 704202
View all MMP-7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMP-7
Met1-Leu264
Accession # Q10738

Specificity

Detects mouse MMP-7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MMP-7, recombinant mouse (rm) MMP‑3, or rmMMP-8 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse MMP‑7 Antibody

Detection of MMP-7 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of MMP-7 in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line treated with 25 ng/mL Recombinant Mouse FGF basic (Catalog # 3139-FB) for 24 hours was stained with Rat Anti-Mouse MMP-7 Monoclonal Antibody (Catalog # MAB7170, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformadehyde and permeabilized with saponin.

Applications for Mouse MMP‑7 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line treated with Recombinant Mouse FGF basic (Catalog # 3139-FB), fixed with paraformadehyde and permeabilized with saponin.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-7

MMP-7 (Matrilysin, 28 kDa) is the smallest member of the matrix metalloproteinases (MMP) family. It is expressed by normal and abnormal epithelial cells and is capable of digesting many proteins of the extracellular matrix.  MMP-7 is implicated in the activation of Plasminogen, FasL, intestinal alpha-Defensin (important in innate host defense), and MMPs-1, -2, and -9, and in the release of TNF-alpha.  Mouse MMP-7 is synthesized with a signal peptide and a propeptide; the preproMMP-7 shows 87% and 70% aa identity with rat and human preproMMP-7, respectively.

Long Name

Matrix Metalloproteinase 7

Alternate Names

Matrilysin, MMP7

Entrez Gene IDs

4316 (Human); 17393 (Mouse)

Gene Symbol

MMP7

UniProt

Q10738

Additional MMP-7 Products

Product Documents for Mouse MMP‑7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse MMP‑7 Antibody

For research use only

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