Detection of MMP-7 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line treated with 25 ng/mL Recombinant Mouse FGF basic (Catalog # 3139-FB) for 24 hours was stained with Rat Anti-Mouse MMP‑7 Monoclonal Antibody (Catalog # MAB7170, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformadehyde and permeabilized with saponin.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MMP-7 (Matrilysin, 28 kDa) is the smallest member of the matrix metalloproteinases (MMP) family. It is expressed by normal and abnormal epithelial cells and is capable of digesting many proteins of the extracellular matrix. MMP-7 is implicated in the activation of Plasminogen, FasL, intestinal alpha-Defensin (important in innate host defense), and MMPs-1, -2, and -9, and in the release of TNF-alpha. Mouse MMP-7 is synthesized with a signal peptide and a propeptide; the preproMMP-7 shows 87% and 70% aa identity with rat and human preproMMP-7, respectively.
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