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Mouse MS4A4B APC-conjugated Antibody

Catalog #: FAB4504A Datasheet / COA / SDS

Discontinued Product

FAB4504A has been discontinued.
View all MS4A4B products.
Detection of MS4A4B in NS0 Mouse Cell Line by Flow Cytometry.
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Mouse MS4A4B APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MS4A4B. Stains mouse MS4A4B transfectants but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 444008
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with mouse MS4A4B
Met1-Val226
Accession # NP_068364
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MS4A4B antibody in NS0 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of MS4A4B in NS0 Mouse Cell Line by Flow Cytometry. NS0 mouse myeloma cell line transfected with mouse MS4A4B and eGFP was stained with either (A) Rat Anti-Mouse MS4A4B APC-conjugated Monoclonal Antibody (Catalog # FAB4504A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MS4A4B

Membrane Spanning 4 domain family, subfamily A4B (MS4A4B), also known as Chandra, is a member of the MS4A family of four-transmembrane proteins that includes CD20. It is expressed in immature CD4-CD8- T cells, NKT cells, CD4+ and CD8+ T cells, CD4+CD25+FoxP3+ Tregs and later in mature Th1 but not Th2 cells. MS4A4B is enriched in lipid rafts of activated T cells, and overexpression enhances Th1 cytokine expression, indicating a possible role in cell signaling.

Long Name
Membrane-spanning 4-domains, Subfamily A, Member 4B
Entrez Gene IDs
60361 (Mouse)
Alternate Names
AI463180; Chandra; Ly116; MS4A4B

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