Mouse Nephrin Antibody

Catalog # Availability Size / Price Qty
AF3159
AF3159-SP
Mouse Nephrin Antibody in Data
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Product Details
Citations (19)
FAQs
Supplemental Products
Reviews (2)

Mouse Nephrin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Nephrin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Nephrin
Gln37-Thr1049
Accession # NP_062332
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Nephrin antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Nephrin in Mouse Kidney. Nephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 µg/mL Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Nephrin antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Nephrin in Mouse Kidney. Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nephrin

Nephrin is a 185 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse Nephrin consists of a 1042 amino acid (aa) extracellular domain (ECD) with eight Ig-like C2-set domains and one fibronectin type III domain, a 22 aa transmembrane segment, and a 156 aa cytoplasmic tail (2, 3). Within the ECD, mouse Nephrin shares 84% and 95% aa sequence identity with human and rat Nephrin, respectively. Usage of the alternate exon 1B results in a distinct N-terminal sequence that lacks a clearly defined signal peptide cleavage site (4). Nephrin is expressed primarily on podocytes in the renal glomerulus and to a lesser extent in the brain and pancreas (3, 5). The 1B isoform is not expressed in the kidney (4). Nephrin localizes to intercellular junctions between podocyte foot processes where it functions as a homophilic adhesion molecule (2, 6). Nephrin is required for formation and maintenance of the slit diaphragm between these processes (7). It associates with Neph1, podicin, P-cadherin, and multiple scaffolding proteins which couple it to the actin cytoskeleton (8-12). Nephrin expression is required for the anti-apoptotic effect of VEGF on podocytes as well as for the ability of podocytes to upregulate Glut1 and Glut4 glucose transporters in response to insulin (13, 14). Nephrin downregulation contributes to diabetic nephropathy, and Nephrin mutations underlie the lethal congenital nephritic syndrome NPHS1 (5, 15).

References
  1. Kawachi, H. et al. (2006) Nephrology 11:274.
  2. Holzman, L.B. et al. (1999) Kidney Int. 56:1481.
  3. Putaala, H. et al. (2000) J. Am. Soc. Nephrol. 11:991.
  4. Beltcheva, O. et al. (2003) J. Am. Soc. Nephrol. 14:352.
  5. Putaala, H. et al. (2001) Hum. Mol. Genet. 10:1.
  6. Khoshnoodi, J. et al. (2003) Am. J. Pathol. 163:2337.
  7. Ruotsalainen, V. et al. (2000) Am. J. Pathol. 157:1905.
  8. Barletta, G.M. et al. (2003) J. Biol. Chem. 278:19266.
  9. Huber, T.B. et al. (2001) J. Biol. Chem. 276:41543.
  10. Lehtonen, S. et al. (2004) Am. J. Pathol. 165:923.
  11. Lehtonen, S. et al. (2005) Proc. Natl. Acad. Sci. USA 102:9814.
  12. Verma, R. et al. (2006) J. Clin. Invest. 116:1346.
  13. Foster, R.R. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F48.
  14. Coward, R.J. et al. (2007) Diabetes 56:1127.
  15. Cooper, M.E. et al. (2002) Semin. Nephrol. 22:393.
Entrez Gene IDs
4868 (Human); 54631 (Mouse); 64563 (Rat)
Alternate Names
CNF; Nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHNCNF; NPHS1; Renal glomerulus-specific cell adhesion receptor

Product Datasheets

Citations for Mouse Nephrin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. The HIV protease inhibitor darunavir prevents kidney injury via HIV-independent mechanisms
    Authors: X Gao, A Rosales, H Karttunen, GM Bommana, B Tandoh, Z Yi, Z Habib, V Agati, W Zhang, MJ Ross
    Sci Rep, 2019;9(1):15857.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Inhibiting 4E-BP1 re-activation represses podocyte cell cycle re-entry and apoptosis induced by adriamycin
    Authors: F Li, X Mao, Q Zhuang, Z Zhao, Z Zhang, H Wu
    Cell Death Dis, 2019;10(3):241.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Effective reconstruction of functional organotypic kidney spheroid for in vitro nephrotoxicity studies
    Authors: HM Kang, JH Lim, KH Noh, D Park, HS Cho, K Susztak, CR Jung
    Sci Rep, 2019;9(1):17610.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction
    Authors: CL Lin, YC Hsu, YT Huang, YH Shih, CJ Wang, WC Chiang, PJ Chang
    EMBO Mol Med, 2019;11(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats
    Authors: T Goto, H Hara, M Sanbo, H Masaki, H Sato, T Yamaguchi, S Hochi, T Kobayashi, H Nakauchi, M Hirabayash
    Nat Commun, 2019;10(1):451.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Change in Renal Glomerular Collagens and Glomerular Filtration Barrier-Related Proteins in a Dextran Sulfate Sodium-Induced Colitis Mouse Model
    Authors: CJ Chang, PC Wang, TC Huang, A Taniguchi
    Int J Mol Sci, 2019;20(6):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Activated renal tubular Wnt/β-catenin signaling�triggers renal inflammation during�overload proteinuria
    Authors: DWL Wong, WH Yiu, KW Chan, Y Li, B Li, SWY Lok, MM Taketo, P Igarashi, LYY Chan, JCK Leung, KN Lai, SCW Tang
    Kidney Int., 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Accelerated Glomerular Cell Senescence in Experimental Lupus Nephritis
    Authors: C Yang, J Xue, N An, XJ Huang, ZH Wu, L Ye, ZH Li, SJ Wang, QJ Pan, D Liang, HF Liu
    Med. Sci. Monit., 2018;24(0):6882-6891.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
    Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Natriuretic peptide receptor guanylyl cyclase-A pathway counteracts glomerular injury evoked by aldosterone through p38 mitogen-activated protein kinase inhibition
    Authors: Y Kato, K Mori, M Kasahara, K Osaki, A Ishii, KP Mori, N Toda, S Ohno, T Kuwabara, T Tokudome, I Kishimoto, MA Saleem, T Matsusaka, K Nakao, M Mukoyama, M Yanagita, H Yokoi
    Sci Rep, 2017;7(0):46624.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Endoplasmic reticulum stress inhibition attenuates hypertensive chronic kidney disease through reduction in proteinuria
    Authors: Z Mohammed-A, C Lu, MK Marway, RE Carlisle, K Ask, D Lukic, JC Krepinsky, JG Dickhout
    Sci Rep, 2017;7(0):41572.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy
    Authors: S Alarcón, W Garrido, C Cappelli, R Suárez, C Oyarzún, C Quezada, R San Martín
    Sci Rep, 2017;7(1):9439.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  13. Alteration of histone H3K4 methylation in glomerular podocytes associated with proteinuria in patients with membranous nephropathy
    BMC Nephrol, 2016;17(1):179.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. Smad3 deficiency protects mice from obesity-induced podocyte injury that precedes insulin resistance.
    Authors: Sun Y, Qu X, Howard V, Dai L, Jiang X, Ren Y, Fu P, Puelles V, Nikolic-Paterson D, Caruana G, Bertram J, Sleeman M, Li J
    Kidney Int, 2015;88(2):286-98.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. TGF-beta-activated kinase 1 is crucial in podocyte differentiation and glomerular capillary formation.
    Authors: Kim S, Lee S, Wang Z, Ding Y, Haque N, Zhang J, Zhou J, Choi M
    J Am Soc Nephrol, 2014;25(9):1966-78.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  16. Morphine induces albuminuria by compromising podocyte integrity.
    Authors: Lan, Xiqian, Rai, Partab, Chandel, Nirupama, Cheng, Kang, Lederman, Rivka, Saleem, Moin A, Mathieson, Peter W, Husain, Mohammad, Crosson, John T, Gupta, Kalpna, Malhotra, Ashwani, Singhal, Pravin C
    PLoS ONE, 2013;8(3):e55748.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Glomerular endothelial surface layer acts as a barrier against albumin filtration.
    Authors: Dane M, van den Berg B, Avramut M, Faas F, van der Vlag J, Rops A, Ravelli R, Koster B, van Zonneveld A, Vink H, Rabelink T
    Am J Pathol, 2013;182(5):1532-40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. Divergent roles of Smad3 and PI3-kinase in murine adriamycin nephropathy indicate distinct mechanisms of proteinuria and fibrogenesis.
    Kidney Int., 2012;82(5):525-36.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.
    Authors: Pierchala BA, Munoz MR, Tsui CC
    Kidney Int., 2010;78(9):868-82.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Mouse Nephrin Antibody

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Mouse Nephrin Antibody
By Anonymous on 06/15/2017
Application: IHC Sample Tested: Kidney (cortex) tissue Species: Mouse

Mouse Nephrin Antibody
By Anonymous on 08/26/2016
Application: WB Sample Tested: Kidney tissue Species: Mouse