Mouse Nephrin Antibody

(10 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Nephrin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human Nephrin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Nephrin
    Gln37-Thr1049
    Accession # NP_062332
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Nephrin (Catalog # 3159-NN)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Nephrin in Mouse Kidney. Nephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 µg/mL Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
Nephrin in Mouse Kidney. Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nephrin

Nephrin is a 185 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse Nephrin consists of a 1042 amino acid (aa) extracellular domain (ECD) with eight Ig-like C2-set domains and one fibronectin type III domain, a 22 aa transmembrane segment, and a 156 aa cytoplasmic tail (2, 3). Within the ECD, mouse Nephrin shares 84% and 95% aa sequence identity with human and rat Nephrin, respectively. Usage of the alternate exon 1B results in a distinct N-terminal sequence that lacks a clearly defined signal peptide cleavage site (4). Nephrin is expressed primarily on podocytes in the renal glomerulus and to a lesser extent in the brain and pancreas (3, 5). The 1B isoform is not expressed in the kidney (4). Nephrin localizes to intercellular junctions between podocyte foot processes where it functions as a homophilic adhesion molecule (2, 6). Nephrin is required for formation and maintenance of the slit diaphragm between these processes (7). It associates with Neph1, podicin, P-cadherin, and multiple scaffolding proteins which couple it to the actin cytoskeleton (8-12). Nephrin expression is required for the anti-apoptotic effect of VEGF on podocytes as well as for the ability of podocytes to upregulate Glut1 and Glut4 glucose transporters in response to insulin (13, 14). Nephrin downregulation contributes to diabetic nephropathy, and Nephrin mutations underlie the lethal congenital nephritic syndrome NPHS1 (5, 15).

  • References:
    1. Kawachi, H. et al. (2006) Nephrology 11:274.
    2. Holzman, L.B. et al. (1999) Kidney Int. 56:1481.
    3. Putaala, H. et al. (2000) J. Am. Soc. Nephrol. 11:991.
    4. Beltcheva, O. et al. (2003) J. Am. Soc. Nephrol. 14:352.
    5. Putaala, H. et al. (2001) Hum. Mol. Genet. 10:1.
    6. Khoshnoodi, J. et al. (2003) Am. J. Pathol. 163:2337.
    7. Ruotsalainen, V. et al. (2000) Am. J. Pathol. 157:1905.
    8. Barletta, G.M. et al. (2003) J. Biol. Chem. 278:19266.
    9. Huber, T.B. et al. (2001) J. Biol. Chem. 276:41543.
    10. Lehtonen, S. et al. (2004) Am. J. Pathol. 165:923.
    11. Lehtonen, S. et al. (2005) Proc. Natl. Acad. Sci. USA 102:9814.
    12. Verma, R. et al. (2006) J. Clin. Invest. 116:1346.
    13. Foster, R.R. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F48.
    14. Coward, R.J. et al. (2007) Diabetes 56:1127.
    15. Cooper, M.E. et al. (2002) Semin. Nephrol. 22:393.
  • Entrez Gene IDs:
    4868 (Human); 54631 (Mouse); 64563 (Rat)
  • Alternate Names:
    CNF; Nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHNCNF; NPHS1; Renal glomerulus-specific cell adhesion receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
    Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Endoplasmic reticulum stress inhibition attenuates hypertensive chronic kidney disease through reduction in proteinuria
    Authors: Z Mohammed-A, C Lu, MK Marway, RE Carlisle, K Ask, D Lukic, JC Krepinsky, JG Dickhout
    Sci Rep, 2017;7(0):41572.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: Western Blot
  3. Natriuretic peptide receptor guanylyl cyclase-A pathway counteracts glomerular injury evoked by aldosterone through p38 mitogen-activated protein kinase inhibition
    Authors: Y Kato, K Mori, M Kasahara, K Osaki, A Ishii, KP Mori, N Toda, S Ohno, T Kuwabara, T Tokudome, I Kishimoto, MA Saleem, T Matsusaka, K Nakao, M Mukoyama, M Yanagita, H Yokoi
    Sci Rep, 2017;7(0):46624.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Alteration of histone H3K4 methylation in glomerular podocytes associated with proteinuria in patients with membranous nephropathy
    BMC Nephrol, 2016;17(1):179.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. Smad3 deficiency protects mice from obesity-induced podocyte injury that precedes insulin resistance.
    Authors: Sun Y, Qu X, Howard V, Dai L, Jiang X, Ren Y, Fu P, Puelles V, Nikolic-Paterson D, Caruana G, Bertram J, Sleeman M, Li J
    Kidney Int, 2015;88(2):286-98.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. TGF-beta-activated kinase 1 is crucial in podocyte differentiation and glomerular capillary formation.
    Authors: Kim S, Lee S, Wang Z, Ding Y, Haque N, Zhang J, Zhou J, Choi M
    J Am Soc Nephrol, 2014;25(9):1966-78.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  7. Glomerular endothelial surface layer acts as a barrier against albumin filtration.
    Authors: Dane M, van den Berg B, Avramut M, Faas F, van der Vlag J, Rops A, Ravelli R, Koster B, van Zonneveld A, Vink H, Rabelink T
    Am J Pathol, 2013;182(5):1532-40.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  8. Morphine induces albuminuria by compromising podocyte integrity.
    Authors: Lan, Xiqian, Rai, Partab, Chandel, Nirupama, Cheng, Kang, Lederman, Rivka, Saleem, Moin A, Mathieson, Peter W, Husain, Mohammad, Crosson, John T, Gupta, Kalpna, Malhotra, Ashwani, Singhal, Pravin C
    PLoS ONE, 2013;8(3):e55748.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Divergent roles of Smad3 and PI3-kinase in murine adriamycin nephropathy indicate distinct mechanisms of proteinuria and fibrogenesis.
    Kidney Int., 2012;82(5):525-36.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.
    Authors: Pierchala BA, Munoz MR, Tsui CC
    Kidney Int., 2010;78(9):868-82.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Acidic

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NorthernLights Guard Mounting Media

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VisUCyte Antigen Retrieval Reagent-Universal

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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 5 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 applications: Immunohistochemistry, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Mouse Nephrin Antibody AF3159
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Excellent
 IHC Mouse Anonymous 06/15/2017
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Image Details
Immunohistochemistry Mouse Nephrin Antibody AF3159
Immunohistochemistry: Mouse Nephrin Antibody [AF3159]

Summary

ApplicationImmunohistochemistry
Sample TestedKidney (cortex) tissue
SpeciesMouse
Western Blot Mouse Nephrin Antibody AF3159
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Excellent
 WB Mouse Anonymous 08/26/2016
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Image Details
Western Blot Mouse Nephrin Antibody AF3159
Western Blot: Mouse Nephrin Antibody [AF3159]

Summary

ApplicationWestern Blot
Sample TestedKidney tissue
SpeciesMouse

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