Mouse Neuropilin-1 Alexa Fluor® 647-conjugated Antibody Summary
Accession # P97333
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Neuropilin‑1 in Mouse CD4+Splenocytes by Flow Cytometry. Mouse CD4+splenocytes were stained with Rabbit Anti-Human/Mouse FoxP3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC8214G) and either (A) Rat Anti-Mouse Neuropilin-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB59941R) or (B) Rat IgG2AAlexa Fluor 647 Isotype Control (Catalog # IC006R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Neuropilin-1 (Nrp‑1, previously Neuropilin; also CD304) is a 130‑140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis. The full-length 923 amino acid (aa) mouse Nrp‑1 contains a 835 aa extracellular domain (ECD) that shares 98% aa identity with rat and 93% aa identity with human, equine, bovine and canine Nrp-1. The ECD contains two N‑terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). The sema domains of Class III secreted semaphorins such as Sema3A bind Nrp‑1 a1a2. Heparin, the heparin-binding forms of VEGF (VEGF165, VEGF-B and VEGF-E), PlGF (PlGF2), and the C‑terminus of Sema3 bind the b1b2 region. Nrp‑1 and Nrp-2 share 48% aa identity within the ECD and can form homo‑ and hetero‑oligomers via interaction of their MAM domains. Neuropilins show partially overlapping expression in neuronal and endothelial cells during development. Both neuropilins act as co‑receptors with multiple molecules, which for Nrp-1 includes Sema3A through Sema3F, Plexin A1 through A4, Plexin B1 and D1. It also interacts with Robo1 and as noted, Nrp-2, as a heterodimer, which binds Sema3C. Finally, it has recently been found to bind miRNA that are complexed to AGO2 in the extracellular space. Both are co‑receptors with VEGF R2 (also called KDR or Flk‑1) for VEGF165 binding. Sema3A signaling can be blocked by VEGF165, which has higher affinity for Npn-1. Nrp-1 is preferentially expressed in developing or remodeling arteries. Nrp‑1 is also expressed on multiple cell types, including keratinocytes, Nrp-1+ T cells, Schwann cells, macrophages, vascular and lymphatic endothelium, breast duct epithelium, hepatic stellate cells, and neural crest cells that give rise to both sensory and autonomic ganglia.
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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