Mouse Neuropilin-1 Antibody

Catalog # Availability Size / Price Qty
MAB59941
MAB59941-SP
Detection of Neuropilin‑1 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (4)
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Mouse Neuropilin-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Neuropilin-1 in ELISAs.
Source
Monoclonal Rat IgG2A Clone # 761704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Neuropilin-1
Phe21-Pro856
Accession # P97333
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Delgoffe, G. et al. (2013) Nature 501:252.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Neuropilin-1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Neuropilin‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rat Anti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB59941) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-1

Neuropilin-1 (Nrp‑1, previously neuropilin; also CD304) is a 130‑140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1‑4). The full-length 923 amino acid (aa) mouse Nrp‑1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with rat and 93% aa identity with human, equine, bovine and canine Nrp-1 (3, 4). The ECD contains two N‑terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). At least one splice variant that diverges at aa 587 and lacks the TM domain has been sequenced (5). This form is potentially a soluble antagonist, based on results from human Nrp‑1 splice variants (1, 6‑8). The sema domains of Class III secreted semaphorins such as Sema3A bind Nrp‑1 a1a2 (9). Heparin, the heparin-binding forms of VEGF (VEGF165, VEGF-B and VEGF-E), PlGF (PlGF2), and the C‑terminus of Sema3 bind the b1b2 region (9, 10). Nrp‑1 and Nrp-2 share 48% aa identity within the ECD and can form homo‑ and hetero‑oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co‑receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (11). However, only Nrp‑1 binds Sema3A, and only Nrp‑2 binds Sema3F (1). Both are co‑receptors with VEGF R2 (also called KDR or Flk‑1) for VEGF165 binding (1). Sema3A signaling can be blocked by VEGF165, which has higher affinity for Npn-1 (12). Nrp-1 is preferentially expressed in developing or remodeling arteries (1, 2). Nrp‑1 is also expressed on dendritic cells and mediates DC‑induced T cell proliferation (13).

References
  1. Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. 
  2. Gu, C. et al. (2003) Dev. Cell 5:45. 
  3. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.
  4. Soker, S. et al. (1998) Cell 92:735.
  5. Entrez accession #EDL11827
  6. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. 
  7. Cackowski, F.C. et al. (2004) Genomics 84:82. 
  8. Rossignol, M. et al. (2000) Genomics 70:211.
  9. Gu, C. et al. (2002) J. Biol. Chem. 277:18069.
  10. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818.
  11. Yaron, A. et al. (2005) Neuron 45:513.
  12. Narazaki, M. and G. Tosato (2006) Blood 107:3892.
  13. Tordjman, R. et al. (2002) Nat. Immunol. 3477.
Entrez Gene IDs
8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)
Alternate Names
BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; Neuropilin1; Neuropilin-1; NP1; NRP; NRP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165R

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Citations for Mouse Neuropilin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1
    Authors: M Leclerc, E Voilin, G Gros, S Corgnac, V de Montpré, P Validire, G Bismuth, F Mami-Choua
    Nat Commun, 2019-07-26;10(1):3345.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  2. CD45Rb-low effector T cells require IL-4 to induce IL-10 in FoxP3 Tregs and to protect mice from inflammation
    Authors: MB Jones, CA Alvarez, JL Johnson, JY Zhou, N Morris, BA Cobb
    PLoS ONE, 2019-05-23;14(5):e0216893.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Blocking
  3. The vimentin intermediate filament network restrains regulatory T-cell suppression of graft-versus-host disease
    Authors: C McDonald-H, JT Muller, M Loschi, G Thangavelu, A Saha, S Kumari, DK Reichenbac, MJ Smith, G Zhang, BH Koehn, J Lin, JS Mitchell, BT Fife, A Panoskalts, CJ Feser, AK Kirchmeier, MJ Osborn, KL Hippen, A Kelekar, JS Serody, LA Turka, DH Munn, H Chi, TA Neubert, ML Dustin, BR Blazar
    J. Clin. Invest., 2018-08-14;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis.
    Authors: Delgoffe, Greg M, Woo, Seng-Ryo, Turnis, Meghan E, Gravano, David M, Guy, Cliff, Overacre, Abigail, Bettini, Matthew, Vogel, Peter, Finkelstein, David, Bonnevier, Jody, Workman, Creg J, Vignali, Dario A
    Nature, 2013-08-04;501(7466):252-6.
    Species: Mouse
    Sample Types:

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