Mouse NKG2A/CD159a Alexa Fluor® 488-conjugated Antibody
Mouse NKG2A/CD159a Alexa Fluor® 488-conjugated Antibody Summary
Accession # Q9Z202
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of NKG2A/CD159a in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 PerCP-conjugated Monoclonal Antibody (Catalog # FAB22252C) and either (A) Rat Anti-Mouse NKG2A/CD159a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB6867G) or (B) Rat IgG2BAlexa Fluor 488 Isotype Control (Catalog # IC013G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
NKG2A, also known as CD159a, is a 244 amino acid (aa), ~38 kDa type II transmembrane glycoprotein with a cytoplasmic ITIM motif and a single extracellular lectin-like domain. Within the extracellular domain (aa 94-244), mouse NKG2A shares 71% and 41% aa sequence identity with rat and human NKG2A, respectively. An isoform lacking aa 98-114 has been described. NKG2A associates with CD94 and is coexpressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its murine ligand, Qa-1b (or HLA-E in humans) on antigen presenting cells.
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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