Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 705829
View all NKG2A/CD159a Primary Antibodies »

Product Specifications

Immunogen

CHO Chinese hamster ovary cell line transfected with mouse NKG2A/CD159a
Accession # Q9Z202

Specificity

Detects mouse NKG2A/CD159a in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse NKG2A/CD159a PE‑conjugated Antibody

Detection of NKG2A/CD159a antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of NKG2A/CD159a in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB22252G) and either (A) Rat Anti-Mouse NKG2A/CD159a PE-conjugated Monoclonal Antibody (Catalog # FAB6867P) or (B) Rat IgG2BPhycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse NKG2A/CD159a PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKG2A/CD159a

NKG2A, also known as CD159a, is a 244 amino acid (aa), ~38 kDa type II transmembrane glycoprotein with a cytoplasmic ITIM motif and a single extracellular lectin-like domain. Within the extracellular domain (aa 94-244), mouse NKG2A shares 71% and 41% aa sequence identity with rat and human NKG2A, respectively. An isoform lacking aa 98-114 has been described. NKG2A associates with CD94 and is coexpressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its murine ligand, Qa-1b (or HLA-E in humans) on antigen presenting cells.

Long Name

Natural Killer G2A

Alternate Names

CD159a, Klrc1, NKG2

Entrez Gene IDs

3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)

Gene Symbol

KLRC1

UniProt

Q9Z202

Additional NKG2A/CD159a Products

Product Documents for Mouse NKG2A/CD159a PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse NKG2A/CD159a PE‑conjugated Antibody

For research use only

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