Mouse NKG2A/CD159a Antibody

Catalog # Availability Size / Price Qty
MAB6867
MAB6867-SP
Detection of NKG2A in HEK293 Human Cell Line transfected with Mouse NKG2A and eGFP by Flow Cytometry
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Mouse NKG2A/CD159a Antibody Summary

Species Reactivity
Mouse
Specificity
Stains mouse NKG2A/CD159a transfectants and not irrelevant transfectants in flow cytometry.
Source
Monoclonal Rat IgG2B Clone # 705829
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with mouse NKG2A/CD159a
Accession # Q9Z202
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of NKG2A antibody in HEK293 Human Cell Line transfected with Mouse NKG2A and eGFP antibody by Flow Cytometry View Larger

Detection of NKG2A in HEK293 Human Cell Line transfected with Mouse NKG2A and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) mouse NKG2A or (B) irrelevant protein, and eGFP, was stained with Rat anti-mouse NKG2A monoclonal antibody (Catalog # MAB6867) followed by Allophycocyanin-conjugated anti-Rat IgG Secondary Antibody (F0113). Quadrant markers were set based on control antibody staining (MAB0061, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Flow Cytometry Detection of NKG2A/CD159a antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of NKG2A/CD159a in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse NKG2A/CD159a Monoclonal Antibody (Catalog # MAB6867) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113) and Rat Anti-Mouse Integrin a2/CD49b PE-conjugated Monoclonal Antibody (Catalog # FAB1740P). Quadrant markers were set based on control antibody staining (Catalog # MAB0061).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2A/CD159a

NKG2A, also known as CD159a, is a 244 amino acid (aa), ~38 kDa type II transmembrane glycoprotein with a cytoplasmic ITIM motif and a single extracellular lectin-like domain. Within the extracellular domain (aa 94-244), mouse NKG2A shares 71% and 41% aa sequence identity with rat and human NKG2A, respectively. An isoform lacking aa 98-114 has been described. NKG2A associates with CD94 and is coexpressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its murine ligand, Qa-1b (or HLA-E in humans) on antigen presenting cells.

Long Name
Natural Killer G2A
Entrez Gene IDs
3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)
Alternate Names
CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B

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