Mouse NKG2A/CD159a APC-conjugated Antibody
Mouse NKG2A/CD159a APC-conjugated Antibody Summary
Accession # Q9Z202
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of NKG2A/CD159a in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB22252G) and either (A) Rat Anti-Mouse NKG2A/CD159a APC-conjugated Monoclonal Antibody (Catalog # FAB6867A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
NKG2A, also known as CD159a, is a 244 amino acid (aa), ~38 kDa type II transmembrane glycoprotein with a cytoplasmic ITIM motif and a single extracellular lectin-like domain. Within the extracellular domain (aa 94-244), mouse NKG2A shares 71% and 41% aa sequence identity with rat and human NKG2A, respectively. An isoform lacking aa 98-114 has been described. NKG2A associates with CD94 and is coexpressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its murine ligand, Qa-1b (or HLA-E in humans) on antigen presenting cells.
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