Mouse NKp46/NCR1 PE-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects mouse NKp46/NCR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human (rh) NKp46 and less than 1% cross-reactivity with rhNKp80 and rhNKp30 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse NKp46/NCR1
    Accession # Q8C567
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of NKp46/NCR1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse NKp46/NCR1 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2225P) and Mouse Anti-Mouse CD161/NK1.1 APC‑conjugated Monoclonal Antibody (Catalog # FAB8319A).View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NKp46/NCR1

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1 (3), is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma (4). Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (5). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (6-8).

  • References:
    1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
    2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
    3. Biassoni, R. et al. (1999) Eur. J. Immunol. 29:1014.
    4. Westgaard, I. et al. (2004) J. Leukoc. Biol. PMID 15356098.
    5. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
    6. Arnon, T. et al. (2004) Blood 103:664.
    7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
    8. Mandelboim, O. et al. (2001) Nature 409:1055.
  • Entrez Gene IDs:
    9437 (Human); 17086 (Mouse)
  • Alternate Names:
    CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Distinct phenotype and function of NK cells in the pancreas of nonobese diabetic mice.
    Authors: Brauner H, Elemans M, Lemos S, Broberger C, Holmberg D, Flodstrom-Tullberg M, Karre K, Hoglund P
    J. Immunol., 2010;184(5):2272-80.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG PE-conjugated Antibody

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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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