|NKX3.1 in NIH‑3T3 Mouse Cell Line. NKX3.1 was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Rat Anti-Mouse NKX3.1 Monoclonal Antibody (Catalog # MAB6475) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
NKX3.1 (Homeobox protein NK-3 homolog A) is a 38 kDa member of the NK-3 homeobox family of transcription factors. It is expressed in mouse palatine and prostatic epithelium, and appears to be regulated by both testosterone and estrogen. NKX3.1 apparently promotes prostate gland development, and acts as a tumor-suppressor/transcriptional repressor by interacting with HDAC-1, potentially inducing IGFBP-3 secretion, and increasing p53 acetylation and half-life. Mouse NKX3.1 is 237 amino acids (aa) in length. It contains a DNA-binding homeodomain (aa 125-184) and undergoes ubiquitination at multiple sites. Over aa 1-124, mouse NKX3.1 shares 77% and 48% aa identity with rat and human NKX3.1, respectively.
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