Mouse NKX3.1 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse NKX3.1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NKX3.1 or rhNKX6.1 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 738907
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse NKX3.1
    Met1-Pro124 (predicted)
    Accession # P97436
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
NKX3.1 in NIH‑3T3 Mouse Cell Line. NKX3.1 was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Rat Anti-Mouse NKX3.1 Monoclonal Antibody (Catalog # MAB6475) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKX3.1

NKX3.1 (Homeobox protein NK-3 homolog A) is a 38 kDa member of the NK-3 homeobox family of transcription factors. It is expressed in mouse palatine and prostatic epithelium, and appears to be regulated by both testosterone and estrogen. NKX3.1 apparently promotes prostate gland development, and acts as a tumor-suppressor/transcriptional repressor by interacting with HDAC-1, potentially inducing IGFBP-3 secretion, and increasing p53 acetylation and half-life. Mouse NKX3.1 is 237 amino acids (aa) in length. It contains a DNA-binding homeodomain (aa 125-184) and undergoes ubiquitination at multiple sites. Over aa 1-124, mouse NKX3.1 shares 77% and 48% aa identity with rat and human NKX3.1, respectively.

  • Long Name:
    NK3 Homeobox 1
  • Entrez Gene IDs:
    4824 (Human); 18095 (Mouse); 305999 (Rat)
  • Alternate Names:
    BAPX2; BAPX2homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A; NK homeobox (Drosophila), family 3, A; NK3 homeobox 1; NK3 transcription factor homolog A; NK3 transcription factor related, locus 1 (Drosophila); NK3 transcription factor related, locus 1; NKX3.1; NKX3.1NKX3; NKX3-1; NKX3A; NKX3ANK homeobox, family 3, A
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