Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all NKX3.1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse NKX3.1
Met1-Pro124
Accession # P97436

Specificity

Detects mouse NKX3.1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse NKX3.1 Antibody

Detection of Mouse NKX3.1 antibody by Western Blot.

Detection of Mouse NKX3.1 by Western Blot.

Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse NKX3.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6475) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NKX3.1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse NKX3.1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: LNCaP human prostate cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: NKX3.1

Nkx3.1 (Homeobox protein NK-3 homolog A) is a 38 kDa member of the NK-3 homeobox family of transcription factors. It is expressed in mouse palatine and prostatic epithelium, and appears to be regulated by both testosterone and estrogen. Nkx3.1 apparently promotes prostate gland development, and acts as a tumor-suppressor/transcriptional repressor by interacting with HDAC-1, potentially inducing IGFBP-3 secretion, and increasing p53 acetylation and half-life. Mouse Nkx3.1 is 237 amino acids (aa) in length. It contains a DNA-binding homeodomain (aa 125-184) and undergoes ubiquitination at multiple sites. Over aa 1-124, mousen Nkx3.1 shares 77% and 48% aa identity with rat and human NKX3.1, respectively.

Long Name

NK3 Homeobox 1

Alternate Names

BAPX2, NKX3-1, NKX3A

Entrez Gene IDs

4824 (Human); 18095 (Mouse); 305999 (Rat)

Gene Symbol

NKX3-1

UniProt

P97436

Additional NKX3.1 Products

Product Documents for Mouse NKX3.1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse NKX3.1 Antibody

For research use only

Customer Reviews for Mouse NKX3.1 Antibody

There are currently no reviews for this product. Be the first to review Mouse NKX3.1 Antibody and earn rewards!

Have you used Mouse NKX3.1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies