Nkx3.1 (Homeobox protein NK-3 homolog A) is a 38 kDa member of the NK-3 homeobox family of transcription factors. It is expressed in mouse palatine and prostatic epithelium, and appears to be regulated by both testosterone and estrogen. Nkx3.1 apparently promotes prostate gland development, and acts as a tumor-suppressor/transcriptional repressor by interacting with HDAC-1, potentially inducing IGFBP-3 secretion, and increasing p53 acetylation and half-life. Mouse Nkx3.1 is 237 amino acids (aa) in length. It contains a DNA-binding homeodomain (aa 125-184) and undergoes ubiquitination at multiple sites. Over aa 1-124, mousen Nkx3.1 shares 77% and 48% aa identity with rat and human NKX3.1, respectively.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse NKX3.1
Met1-Pro124
Accession # P97436
Met1-Pro124
Accession # P97436
Specificity
Detects mouse NKX3.1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse NKX3.1 Antibody
Detection of Mouse NKX3.1 by Western Blot.
Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse NKX3.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6475) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NKX3.1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Mouse NKX3.1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: LNCaP human prostate cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: LNCaP human prostate cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: NKX3.1
Long Name
NK3 Homeobox 1
Alternate Names
BAPX2, NKX3-1, NKX3A
Gene Symbol
NKX3-1
UniProt
Additional NKX3.1 Products
Product Documents for Mouse NKX3.1 Antibody
Product Specific Notices for Mouse NKX3.1 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
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- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars