Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 202604
View all Nogo Receptor/NgR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse NgR
Cys27-Ser447
Accession # Q99PI8

Specificity

Detects mouse NgR in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human NgR.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse Nogo Receptor/NgR Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Nogo Receptor/NgR Fc Chimera (Catalog # 1440-NG)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nogo Receptor/NgR

NgR,also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored proteinthat belongs to the family of leucine-rich repeat proteins. NgR plays an essential role inmediating axon growth inhibition induced by the myelin-derived proteins Nogo, myelinassociatedglycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp). Uponligand binding, NgR associates with and activates the p75 neurotrophin receptor(p75NTR), a tumor necrosis factor superfamily member (TNFRSF16).

Long Name

Nogo-66 Receptor/Reticulon 4 Receptor

Alternate Names

NgR1, NogoR, RTN4R

Entrez Gene IDs

65078 (Human); 65079 (Mouse)

Gene Symbol

RTN4R

UniProt

Q99PI8

Additional Nogo Receptor/NgR Products

Product Documents for Mouse Nogo Receptor/NgR Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Nogo Receptor/NgR Antibody

For research use only

Related Research Areas

Glial Lineage Markers

Citations for Mouse Nogo Receptor/NgR Antibody

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