Mouse Notch-2 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Notch-2 in direct ELISAs. In direct ELISAs, 50-100% cross-reactivity with recombinant human (rh) Notch-2 and no cross-reactivity with rhNotch-1, -2, -3, recombinant mouse (rm) Notch-1, rmNotch-3, recombinant rat (rr) Notch2, or rrDLL1 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 605724
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Notch-2
    Leu26-Val528 (predicted)
    Accession # O35516
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Notch‑2 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Mouse Notch‑2 Monoclonal Antibody (Catalog # MAB5196, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Notch-2

Notch-2 (also Motch-2) is a 280-300 kDa member of the notch family of transmembrane (TM) proteins.  It is found on multiple cell types and exhibits multiple functions.  On CD8+ T cells, it augments cytotoxic activity; on pre-B cells, it drives B1 B cell development; on endothelial cells, it induces apoptosis; and on proerythroblasts, it promotes proliferation.  Mature mouse Notch-2 is a 2445 amino acid (aa) type I TM glycoprotein. It undergoes Golgi processing to generate a 180-200 kDa extracellular domain (ECD) (aa 26-1606) possibly covalently-linked to a 100-110 kDa membrane bound C-terminal segment (aa 1607-2470).  Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) that serves as a potential nuclear transcription factor.  There are three potential splice variants.  One shows a 32 aa substitution for aa 293-2470, a second contains a 13 aa substitution for aa 1-1954, and a third possesses a five aa substitution for aa 224-227 coupled to an 11 aa substitution for aa 1704-1713.  Over aa 26-528, mouse Notch-2 shares 96% and 94% aa identity with rat and human Notch-2, respectively.   

  • Entrez Gene IDs:
    4853 (Human); 29492 (Rat)
  • Alternate Names:
    AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Isotype Control

Ctrl MAB005 37  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG1 Isotype Control

Ctrl MAB005R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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