Mouse Oncostatin M/OSM Antibody (824157)

R&D Systems | Catalog # MAB4951

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Neutralization

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 824157
View all Oncostatin M/OSM Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Oncostatin M/OSM
Ala24-Arg206
Accession # P53347

Specificity

Detects mouse Oncostatin M/OSM in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse Oncostatin M/OSM Antibody (824157)

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Mouse Oncostatin M/OSM Antibody

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Mouse Oncostatin M/OSM Antibody

Recombinant Mouse Oncostatin M/OSM (Catalog # 495-MO) stimulates proliferation in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Oncostatin M/OSM (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Oncostatin M/OSM Monoclonal Antibody (Catalog # MAB4951). The ND50 is typically 0.2-1.2 µg/mL.

Applications for Mouse Oncostatin M/OSM Antibody (824157)

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the NIH‑3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.2-1.2 µg/mL in the presence of 1 ng/mL Recombinant Mouse Oncostatin M/OSM.

Reviewed Applications

Read 1 review rated 4 using MAB4951 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Oncostatin M/OSM

Oncostatin M (OSM) is a member of a cytokine subfamily that includes IL-6, IL-11, LIF, CNTF, and Cardiotrophin-1. These cytokines have overlapping biological functions and shared receptor components. Mouse OSM was cloned and identified as an immediate early gene induced in various myeloid and lymphoid cell lines by a subset of cytokines including IL-2, IL-3, GM-CSF, and Erythropoietin. The mouse OSM cDNA encodes a 263 amino acid residue precursor protein that shows 48% identity with human OSM. Similar to human OSM, the C-terminal region of mouse OSM contains a highly charged region. Deletion of this C-terminal region appears to be essential for the formation of biologically active mouse OSM. The biological activity of human OSM has been shown to be mediated either by the LIF/OSM receptor complex composed of gp130 and LIF R alpha or by a human OSM specific receptor composed of gp130 and OSM R alpha. It remains to be determined if the biological activities of mouse OSM can also be mediated by both receptor complexes in mouse cells.

References

  1. Yoshimura, A. et al. (1996) EMBO J. 15:1055.
  2. Ray, P. et al. (1996) Endocrinology 137:1151.
  3. Rose, T.M. and A.G. Bruce (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, editor, Oxford University Press, New York, p. 127.

Alternate Names

MGC20461, oncostatin M, oncostatin-M

Entrez Gene IDs

5008 (Human); 18413 (Mouse)

Gene Symbol

OSM

UniProt

P53347

Additional Oncostatin M/OSM Products

Product Documents for Mouse Oncostatin M/OSM Antibody (824157)

Certificate of Analysis

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Product Specific Notices for Mouse Oncostatin M/OSM Antibody (824157)

For research use only

Citations for Mouse Oncostatin M/OSM Antibody (824157)

Customer Reviews for Mouse Oncostatin M/OSM Antibody (824157) (1)

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  • Mouse Oncostatin M/OSM Antibody
    Name: Laure FAVOT LAFORGE
    Application: Immunocytochemistry
    Sample Tested: Mouse Ear Skin (frozen section)
    Species: Mouse
    Verified Customer | Posted 03/30/2017
    Frozen slide Fixation: faraformaldhehyde 4% mouse ear skin injected with OSM adenovirus
    Mouse Oncostatin M/OSM Antibody (824157) MAB4951

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