Mouse Osteoactivin/GPNMB Antibody

Name: Mouse Osteoactivin/GPNMB Antibody
Brand: R&D Systems

(6 citations)

Datasheet / CoA / SDS

Neutralization

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Product Details

Citations (6)

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Species Reactivity

Mouse
Specificity

Detects mouse Osteoactivin/GPNMB in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Osteoactivin, rhSyndecan-4, and recombinant mouse Syndecan-4 is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Osteoactivin/GPNMB
Lys23-Asn502
Accession # Q8BVA0
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC)

Mouse Osteoactivin/GPNMB Sandwich Immunoassay

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Mouse Osteoactivin/GPNMB Antibody (Catalog # AF2330 )

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Mouse Osteoactivin/GPNMB Biotinylated Antibody (Catalog # BAF2330 )

ELISA Standard

Recombinant Mouse Osteoactivin/GPNMB Fc Chimera Protein, CF (Catalog # 2330-AC )

Neutralization

Measured by its ability to neutralize Osteoactivin/GPNMB-mediated adhesion of the SVEC4‑10 mouse vascular endothelial cell line. The Neutralization Dose (ND₅₀) is typically 0.2-0.8 µg/mL in the presence of 0.25 µg/mL Recombinant Mouse Osteoactivin/GPNMB Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization

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Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody. Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC), immobilized onto a microplate previously coated with Goat Anti-Mouse IgG Fc (Catalog # G-202-C), supports the adhesion of the SVEC4‑10 mouse vascular endothelial cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Osteoactivin/
GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330). The ND₅₀ is typically
0.2‑0.8 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteoactivin/GPNMB

Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.

References:
1. Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
2. Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
3. Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.

Long Name:

Glycoprotein Non-Metastatic Melanoma Protein B

Entrez Gene IDs:

10457 (Human); 93695 (Mouse)

Alternate Names:

DC-HIL; glycoprotein (transmembrane) nmb; glycoprotein NMB; GPNMB; HGFIN; HGFINglycoprotein nmb-like protein; NMBosteoactivin; Osteoactivin; Transmembrane glycoprotein HGFIN; transmembrane glycoprotein NMB

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species	Applications	Sample Type
HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution Authors: MJ Tol, MJC van der Li, TL Gabriel, JJ Hagen, S Scheij, T Veenendaal, J Klumperman, WE Donker-Koo, AJ Verhoeven, H Overkleeft, JM Aerts, CA Argmann, M van Eijk Autophagy, 2018;0(0):1-13. Species: Mouse Sample Type: Cell Lysates Application: WB Gpnmb Is a Potential Marker for the Visceral Pathology in Niemann-Pick Type C Disease. Authors: Marques A, Gabriel T, Aten J, van Roomen C, Ottenhoff R, Claessen N, Alfonso P, Irun P, Giraldo P, Aerts J, van Eijk M PLoS ONE, 2016;11(1):e0147208. Species: Mouse Sample Type: Cell Lysates Application: WB Lysosomal stress in obese adipose tissue macrophages contributes to MITF-dependent Gpnmb induction. Authors: Gabriel T, Tol M, Ottenhof R, van Roomen C, Aten J, Claessen N, Hooibrink B, de Weijer B, Serlie M, Argmann C, van Elsenburg L, Aerts J, van Eijk M Diabetes, 2014;63(10):3310-23. Species: Mouse Sample Type: Cell Lysates Application: WB A role for Na+,K+-ATPase alpha1 in regulating Rab27a localisation on melanosomes. Authors: Booth A, Tarafder A, Hume A, Recchi C, Seabra M PLoS ONE, 2014;9(7):e102851. Species: Mouse Sample Type: Cell Lysates Application: WB Dependency of intraocular pressure elevation and glaucomatous changes in DBA/2J and DBA/2J-Rj mice. Authors: Scholz M, Buder T, Seeber S, Adamek E, Becker CM, Lutjen-Drecoll E Invest. Ophthalmol. Vis. Sci., 2008;49(2):613-21. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Osteoactivin is a novel osteoclastic protein and plays a key role in osteoclast differentiation and activity. Authors: Sheng MH, Wergedal JE, Mohan S, Lau KH FEBS Lett., 2008;582(10):1451-8. Species: Mouse Sample Type: Whole Cells Application: Neut

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Isotype Controls

Description Application Cat# Citations Images

Normal Goat IgG Control

Ctrl AB-108-C 203

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Mouse Osteoactivin/GPNMB Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Normal Goat IgG Control

Reconstitution Buffer 1 (PBS)

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Goat IgG VisUCyte HRP Polymer

Donkey Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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