Mouse Osteoactivin/GPNMB Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Osteoactivin/GPNMB in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Osteoactivin, rhSyndecan-4, and recombinant mouse Syndecan-4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Osteoactivin/GPNMB
    Lys23-Asn502
    Accession # Q8BVA0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC)
    • Mouse Osteoactivin/GPNMB Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse Osteoactivin/GPNMB Antibody (Catalog # AF2330)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Osteoactivin/GPNMB Biotinylated Antibody (Catalog # BAF2330)
  • ELISA Standard
     
    Recombinant Mouse Osteoactivin/GPNMB Fc Chimera Protein, CF (Catalog # 2330-AC)
  • Neutralization
    Measured by its ability to neutralize Osteoactivin/GPNMB-mediated adhesion of the SVEC4‑10 mouse vascular endothelial cell line. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.25 µg/mL Recombinant Mouse Osteoactivin/GPNMB Fc Chimera.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody. Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC), immobilized onto a microplate previously coated with Goat Anti-Mouse IgG Fc (Catalog # G-202-C), supports the adhesion of the SVEC4‑10 mouse vascular endothelial cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Osteoactivin/
GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330). The ND50 is typically
0.2‑0.8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteoactivin/GPNMB

Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.

  • References:
    1. Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
    2. Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
    3. Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.
  • Long Name:
    Glycoprotein Non-Metastatic Melanoma Protein B
  • Entrez Gene IDs:
    10457 (Human); 93695 (Mouse)
  • Alternate Names:
    DC-HIL; glycoprotein (transmembrane) nmb; glycoprotein NMB; GPNMB; HGFIN; HGFINglycoprotein nmb-like protein; NMBosteoactivin; Osteoactivin; Transmembrane glycoprotein HGFIN; transmembrane glycoprotein NMB
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Gpnmb Is a Potential Marker for the Visceral Pathology in Niemann-Pick Type C Disease.
    Authors: Marques A, Gabriel T, Aten J, van Roomen C, Ottenhoff R, Claessen N, Alfonso P, Irun P, Giraldo P, Aerts J, van Eijk M
    PLoS ONE, 2016;11(1):e0147208.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Lysosomal stress in obese adipose tissue macrophages contributes to MITF-dependent Gpnmb induction.
    Authors: Gabriel T, Tol M, Ottenhof R, van Roomen C, Aten J, Claessen N, Hooibrink B, de Weijer B, Serlie M, Argmann C, van Elsenburg L, Aerts J, van Eijk M
    Diabetes, 2014;63(10):3310-23.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. A role for Na+,K+-ATPase alpha1 in regulating Rab27a localisation on melanosomes.
    Authors: Booth A, Tarafder A, Hume A, Recchi C, Seabra M
    PLoS ONE, 2014;9(7):e102851.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Dependency of intraocular pressure elevation and glaucomatous changes in DBA/2J and DBA/2J-Rj mice.
    Authors: Scholz M, Buder T, Seeber S, Adamek E, Becker CM, Lutjen-Drecoll E
    Invest. Ophthalmol. Vis. Sci., 2008;49(2):613-21.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Osteoactivin is a novel osteoclastic protein and plays a key role in osteoclast differentiation and activity.
    Authors: Sheng MH, Wergedal JE, Mohan S, Lau KH
    FEBS Lett., 2008;582(10):1451-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Donkey Anti-Goat IgG Biotinylated Antibody

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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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