Mouse Osteoactivin/GPNMB Antibody
Mouse Osteoactivin/GPNMB Antibody Summary
Lys23-Asn502
Accession # Q8BVA0
Applications
Mouse Osteoactivin/GPNMB Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
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Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody. Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC), immobilized onto a microplate previously coated with Goat Anti-Mouse IgG Fc (Catalog # G-202-C), supports the adhesion of the SVEC4-10 mouse vascular endothelial cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Osteoactivin/ GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330). The ND50 is typically 0.2-0.8 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteoactivin/GPNMB
Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.
- Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
- Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
- Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.
Product Datasheets
Citations for Mouse Osteoactivin/GPNMB Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice
Authors: MJC van der Li, J Aten, ARA Marques, ISE Waas, PWB Larsen, N Claessen, NN van der We, R Ottenhoff, M van Eijk, JMFG Aerts
International Journal of Molecular Sciences, 2021;22(5):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations
Authors: M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar
Acta Neuropathol Commun, 2020;8(1):163.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Glycoprotein NMB: a novel Alzheimer's disease associated marker expressed in a subset of activated microglia
Authors: M Hüttenrauc, I Ogorek, H Klafki, M Otto, C Stadelmann, S Weggen, J Wiltfang, O Wirths
Acta Neuropathol Commun, 2018;6(1):108.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution
Authors: MJ Tol, MJC van der Li, TL Gabriel, JJ Hagen, S Scheij, T Veenendaal, J Klumperman, WE Donker-Koo, AJ Verhoeven, H Overkleeft, JM Aerts, CA Argmann, M van Eijk
Autophagy, 2018;0(0):1-13.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Gpnmb Is a Potential Marker for the Visceral Pathology in Niemann-Pick Type C Disease.
Authors: Marques A, Gabriel T, Aten J, van Roomen C, Ottenhoff R, Claessen N, Alfonso P, Irun P, Giraldo P, Aerts J, van Eijk M
PLoS ONE, 2016;11(1):e0147208.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Glycoprotein nonmetastatic melanoma protein B extracellular fragment shows neuroprotective effects and activates the PI3K/Akt and MEK/ERK pathways via the Na+/K+-ATPase.
Authors: Yoko Ono, Kazuhiro Tsuruma, Masafumi Takata, Masamitsu Shimazawa, Hideaki Hara
Scientific Reports, 2016;0(0):2045-2322.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
A role for Na+,K+-ATPase alpha1 in regulating Rab27a localisation on melanosomes.
Authors: Booth A, Tarafder A, Hume A, Recchi C, Seabra M
PLoS ONE, 2014;9(7):e102851.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Lysosomal stress in obese adipose tissue macrophages contributes to MITF-dependent Gpnmb induction.
Authors: Gabriel T, Tol M, Ottenhof R, van Roomen C, Aten J, Claessen N, Hooibrink B, de Weijer B, Serlie M, Argmann C, van Elsenburg L, Aerts J, van Eijk M
Diabetes, 2014;63(10):3310-23.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Osteoactivin is a novel osteoclastic protein and plays a key role in osteoclast differentiation and activity.
Authors: Sheng MH, Wergedal JE, Mohan S, Lau KH
FEBS Lett., 2008;582(10):1451-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Dependency of intraocular pressure elevation and glaucomatous changes in DBA/2J and DBA/2J-Rj mice.
Authors: Scholz M, Buder T, Seeber S, Adamek E, Becker CM, Lutjen-Drecoll E
Invest. Ophthalmol. Vis. Sci., 2008;49(2):613-21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
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