Mouse Osteoactivin/GPNMB Antibody

AF2330 6 Citations Datasheet / COA / SDS

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AF2330
AF2330-SP

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Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody.

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Osteoactivin/GPNMB Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Osteoactivin/GPNMB in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Osteoactivin, rhSyndecan-4, and recombinant mouse Syndecan-4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Osteoactivin/GPNMB
Lys23-Asn502
Accession # Q8BVA0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC)

Mouse Osteoactivin/GPNMB Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Mouse Osteoactivin/GPNMB Biotinylated Antibody (Catalog # BAF2330)

Standard: Recombinant Mouse Osteoactivin/GPNMB Fc Chimera Protein, CF (Catalog # 2330-AC)

Neutralization

Measured by its ability to neutralize Osteoactivin/GPNMB-mediated adhesion of the SVEC4‑10 mouse vascular endothelial cell line. The Neutralization Dose (ND₅₀) is typically 0.2-0.8 µg/mL in the presence of 0.25 µg/mL Recombinant Mouse Osteoactivin/GPNMB Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization

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Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody. Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC), immobilized onto a microplate previously coated with Goat Anti-Mouse IgG Fc (Catalog # G-202-C), supports the adhesion of the SVEC4‑10 mouse vascular endothelial cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Osteoactivin/
GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330). The ND₅₀ is typically
0.2‑0.8 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteoactivin/GPNMB

Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.

References

Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.

Long Name

Glycoprotein Non-Metastatic Melanoma Protein B

Entrez Gene IDs

10457 (Human); 93695 (Mouse)

Alternate Names

DC-HIL; glycoprotein (transmembrane) nmb; glycoprotein NMB; GPNMB; HGFIN; HGFINglycoprotein nmb-like protein; NMBosteoactivin; Osteoactivin; Transmembrane glycoprotein HGFIN; transmembrane glycoprotein NMB

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Citations for Mouse Osteoactivin/GPNMB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution
Authors: MJ Tol, MJC van der Li, TL Gabriel, JJ Hagen, S Scheij, T Veenendaal, J Klumperman, WE Donker-Koo, AJ Verhoeven, H Overkleeft, JM Aerts, CA Argmann, M van Eijk
Autophagy, 2018;0(0):1-13.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB
Gpnmb Is a Potential Marker for the Visceral Pathology in Niemann-Pick Type C Disease.
Authors: Marques A, Gabriel T, Aten J, van Roomen C, Ottenhoff R, Claessen N, Alfonso P, Irun P, Giraldo P, Aerts J, van Eijk M
PLoS ONE, 2016;11(1):e0147208.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB
A role for Na+,K+-ATPase alpha1 in regulating Rab27a localisation on melanosomes.
Authors: Booth A, Tarafder A, Hume A, Recchi C, Seabra M
PLoS ONE, 2014;9(7):e102851.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB
Lysosomal stress in obese adipose tissue macrophages contributes to MITF-dependent Gpnmb induction.
Authors: Gabriel T, Tol M, Ottenhof R, van Roomen C, Aten J, Claessen N, Hooibrink B, de Weijer B, Serlie M, Argmann C, van Elsenburg L, Aerts J, van Eijk M
Diabetes, 2014;63(10):3310-23.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB
Osteoactivin is a novel osteoclastic protein and plays a key role in osteoclast differentiation and activity.
Authors: Sheng MH, Wergedal JE, Mohan S, Lau KH
FEBS Lett., 2008;582(10):1451-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Neut
Dependency of intraocular pressure elevation and glaucomatous changes in DBA/2J and DBA/2J-Rj mice.
Authors: Scholz M, Buder T, Seeber S, Adamek E, Becker CM, Lutjen-Drecoll E
Invest. Ophthalmol. Vis. Sci., 2008;49(2):613-21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Paraffin-embedded