Mouse Osteoactivin/GPNMB Antibody

Catalog # Availability Size / Price Qty
Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody.
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Product Details
Citations (10)
Supplemental Products

Mouse Osteoactivin/GPNMB Antibody Summary

Species Reactivity
Detects mouse Osteoactivin/GPNMB in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Osteoactivin, rhSyndecan-4, and recombinant mouse Syndecan-4 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Osteoactivin/GPNMB
Accession # Q8BVA0
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC)

Mouse Osteoactivin/GPNMB Sandwich Immunoassay

Recommended Concentration
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Osteoactivin/GPNMB Biotinylated Antibody (Catalog # BAF2330)

Standard: Recombinant Mouse Osteoactivin/GPNMB Fc Chimera Protein, CF (Catalog # 2330-AC)

Measured by its ability to neutralize Osteoactivin/GPNMB-mediated adhesion of the SVEC4‑10 mouse vascular endothelial cell line. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.25 µg/mL Recombinant Mouse Osteoactivin/GPNMB Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody. View Larger

Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody. Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC), immobilized onto a microplate previously coated with Goat Anti-Mouse IgG Fc (Catalog # G-202-C), supports the adhesion of the SVEC4-10 mouse vascular endothelial cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Osteoactivin/ GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330). The ND50 is typically 0.2-0.8 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteoactivin/GPNMB

Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.

  1. Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
  2. Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
  3. Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.
Long Name
Glycoprotein Non-Metastatic Melanoma Protein B
Entrez Gene IDs
10457 (Human); 93695 (Mouse); 113955 (Rat); 102133517 (Cynomolgus Monkey)
Alternate Names
DC-HIL; glycoprotein (transmembrane) nmb; glycoprotein NMB; GPNMB; HGFIN; HGFINglycoprotein nmb-like protein; NMBosteoactivin; Osteoactivin; Transmembrane glycoprotein HGFIN; transmembrane glycoprotein NMB

Product Datasheets

Citations for Mouse Osteoactivin/GPNMB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice
    Authors: MJC van der Li, J Aten, ARA Marques, ISE Waas, PWB Larsen, N Claessen, NN van der We, R Ottenhoff, M van Eijk, JMFG Aerts
    International Journal of Molecular Sciences, 2021;22(5):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations
    Authors: M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar
    Acta Neuropathol Commun, 2020;8(1):163.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  3. Glycoprotein NMB: a novel Alzheimer's disease associated marker expressed in a subset of activated microglia
    Authors: M Hüttenrauc, I Ogorek, H Klafki, M Otto, C Stadelmann, S Weggen, J Wiltfang, O Wirths
    Acta Neuropathol Commun, 2018;6(1):108.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution
    Authors: MJ Tol, MJC van der Li, TL Gabriel, JJ Hagen, S Scheij, T Veenendaal, J Klumperman, WE Donker-Koo, AJ Verhoeven, H Overkleeft, JM Aerts, CA Argmann, M van Eijk
    Autophagy, 2018;0(0):1-13.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Gpnmb Is a Potential Marker for the Visceral Pathology in Niemann-Pick Type C Disease.
    Authors: Marques A, Gabriel T, Aten J, van Roomen C, Ottenhoff R, Claessen N, Alfonso P, Irun P, Giraldo P, Aerts J, van Eijk M
    PLoS ONE, 2016;11(1):e0147208.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Glycoprotein nonmetastatic melanoma protein B extracellular fragment shows neuroprotective effects and activates the PI3K/Akt and MEK/ERK pathways via the Na+/K+-ATPase.
    Authors: Yoko Ono, Kazuhiro Tsuruma, Masafumi Takata, Masamitsu Shimazawa, Hideaki Hara
    Scientific Reports, 2016;0(0):2045-2322.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. A role for Na+,K+-ATPase alpha1 in regulating Rab27a localisation on melanosomes.
    Authors: Booth A, Tarafder A, Hume A, Recchi C, Seabra M
    PLoS ONE, 2014;9(7):e102851.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Lysosomal stress in obese adipose tissue macrophages contributes to MITF-dependent Gpnmb induction.
    Authors: Gabriel T, Tol M, Ottenhof R, van Roomen C, Aten J, Claessen N, Hooibrink B, de Weijer B, Serlie M, Argmann C, van Elsenburg L, Aerts J, van Eijk M
    Diabetes, 2014;63(10):3310-23.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Osteoactivin is a novel osteoclastic protein and plays a key role in osteoclast differentiation and activity.
    Authors: Sheng MH, Wergedal JE, Mohan S, Lau KH
    FEBS Lett., 2008;582(10):1451-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Dependency of intraocular pressure elevation and glaucomatous changes in DBA/2J and DBA/2J-Rj mice.
    Authors: Scholz M, Buder T, Seeber S, Adamek E, Becker CM, Lutjen-Drecoll E
    Invest. Ophthalmol. Vis. Sci., 2008;49(2):613-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P


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