Mouse Osteoactivin/GPNMB Biotinylated Antibody

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Product Details
Citations (1)
Supplemental Products

Mouse Osteoactivin/GPNMB Biotinylated Antibody Summary

Species Reactivity
Detects mouse Osteoactivin/GPNMB in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Osteoactivin, rhSyndecan-4, and recombinant mouse Syndecan-4 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Osteoactivin/GPNMB
Accession # NP_444340
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC)

Mouse Osteoactivin/GPNMB Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Osteoactivin/GPNMB Antibody (Catalog # AF2330)

Standard: Recombinant Mouse Osteoactivin/GPNMB Fc Chimera Protein, CF (Catalog # 2330-AC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteoactivin/GPNMB

Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.

  1. Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
  2. Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
  3. Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.
Long Name
Glycoprotein Non-Metastatic Melanoma Protein B
Entrez Gene IDs
10457 (Human); 93695 (Mouse); 113955 (Rat); 102133517 (Cynomolgus Monkey)
Alternate Names
DC-HIL; glycoprotein (transmembrane) nmb; glycoprotein NMB; GPNMB; HGFIN; HGFINglycoprotein nmb-like protein; NMBosteoactivin; Osteoactivin; Transmembrane glycoprotein HGFIN; transmembrane glycoprotein NMB

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Citation for Mouse Osteoactivin/GPNMB Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Intracerebroventricular Treatment with 2-Hydroxypropyl-&beta-Cyclodextrin Decreased Cerebellar and Hepatic Glycoprotein Nonmetastatic Melanoma Protein B (GPNMB) Expression in Niemann-Pick Disease Type C Model Mice
    Authors: M Fukaura, Y Ishitsuka, S Shirakawa, N Ushihama, Y Yamada, Y Kondo, T Takeo, N Nakagata, K Motoyama, T Higashi, H Arima, Y Kurauchi, T Seki, H Katsuki, K Higaki, M Matsuo, T Irie
    International Journal of Molecular Sciences, 2021-01-05;22(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC


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