Mouse OX40 Ligand/TNFSF4 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM12361

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Mouse OX40 Ligand/TNFSF4 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse OX40 Ligand/TNFSF4 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) OX40, recombinant human (rh) OX40 Ligand, rmFas Ligand, rmLIGHT, or rhLIGHT is observed.
Source
Monoclonal Rat IgG2B Clone # 182603
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse OX40 Ligand/TNFSF4
Gln49-Leu198
Accession # P43488
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Mouse OX40 Ligand/TNFSF4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Mouse OX40 Ligand/TNFSF4 Antibody (Catalog # MAB12362)

Standard: Recombinant Mouse OX40 Ligand/TNFSF4 Protein (Catalog # 1236-OX)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Murine OX40L cDNA encodes a 198 amino acid (aa) protein comprised of a 28 aa N-terminal cytoplasmic domain, a 20 aa transmembrane segment, and a 150 aa C-terminal extracellular domain (1). Human and mouse OX40L share 46% sequence identity at the amino acid level (1). OX40L is expressed on activated antigen presenting cells such as B cells, macrophages, dendritic cells, and on endothelial cells at the site of inflammation. The receptor for OX40L is OX40 (CD134) which is expressed predominantly on activated CD4T cells. Expression of OX40 is transient following engagement of T cell receptors (2). Ligation of OX40L by OX40 stimulates proliferation and differentiation of activated B cells, and increases immunoglobulin secretion (3, 4). The expression of OX40L on B cells is upregulated by CD40 ligation (3). Engagement of the OX40‑OX40L system has co-stimulatory effects on T cells by stimulating the production of cytokines by T helper cells and increasing the survival of memory T cells (2, 5). Blocking of the OX40‑OX40L interaction in vitro inhibits co-stimulation resulting in decreased T cell proliferation and adhesion of T cells to endothelial cells. Inhibition of the OX40‑OX40L interaction in disease models has beneficial effects in acute graft-versus-host disease, inflammatory bowel disease and decreases the development of collagen-induced arthritis and experimental leishmaniasis (6).

References
  1. Baum, P.R. et al. (1994) EMBO J. 13:3992.
  2. Gramaglia, I. et al. (1999) J. Immunol. 161:6510.
  3. Stuber, E. et al. (1995) Immunity 2:507.
  4. Malstrom, V. et al. (2001) J. Immunol. 166:6972.
  5. Maxwell, J.R. et al. (2000) J. Immunol. 164:107.
  6. Weinberg, A.D. (2002) Trends Immunol. 23:102.
Entrez Gene IDs
7292 (Human); 22164 (Mouse); 89814 (Rat)
Alternate Names
CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1

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