Mouse PDGFRL Antibody

Catalog # Availability Size / Price Qty
MAB9739-100
MAB9739-SP
Cell Proliferation Induced by PDGFRL and Neutralization by Mouse PDGFRL Antibody.
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Mouse PDGFRL Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PDGFRL in direct ELISAs.
Source
Monoclonal Rat IgG2B Clone # 1010639
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell HEK293-derived mouse PDGFRL
Gln22-Ser375
Accession # Q6PE55
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize PDGFRL-induced proliferation in the HCT-116 human colorectal carcinoma cell line. The Neutralization Dose (ND50) is typically 4-24 µg/mL in the presence of 10 μg/mL Recombinant Mouse PDGFRL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by PDGFRL and Neutralization by Mouse PDGFRL Antibody. View Larger

Cell Proliferation Induced by PDGFRL and Neutralization by Mouse PDGFRL Antibody. Recombinant Mouse PDGFRL (Catalog # 9739-PR) stimulates proliferation in the HCT-116 human colorectal carcinoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse PDGFRL (10 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse PDGFRL Monoclonal Antibody (Catalog # MAB9739). The ND50 is typically 4-24 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGFRL

Platelet-derived growth factor receptor-like protein (PDGFRL) is a 67 kDa glycoprotein protein consisting of two Ig-like C2-type domains. By sequence similarity, mouse PDGFRL is 90% similar to the human version and 95% similar to that of the rat. It is a secreted protein related to the class III subfamily of receptor tyrosine kinases (RTK), the platelet-derived growth factor receptors (1-5). The high frequency of loss of heterozygosity (LOH) near the gene of PDGFRL indicated in several studies that PDGFRL can be a tumor suppressor gene (TSG) in breast cancer (6-9), colorectal cancer, prostate cancer (10), non-small cell lung cancers (11) and hepatocellular carcinoma (12). A variant of PDGFRL is found to play a role in the development of Behçet disease, a complex immunoregulatory disease (13). The autoimmune role of PDGFRL is also supported by its up-regulation in a mouse model for Rheumatoid Arthritis (14). Another study also showed that PDGFRL may play a role in chondrocyte proliferation and differentiation (15).

References
  1. Guo, F-J. et al. (2010). World J Gastroenterol. 16:1465.
  2. Andrae, J. et al. (2008) Genes Dev. 22:1276.
  3. Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
  4. Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
  5. Matsui, T. et al. (1989) Science 243:800.
  6. Seitz, S. et al. (2006). Genes Chromosomes Cancer 45:612.
  7. Yaremko, M.L. et al. (1996). Genes, Chromosome Cancer 16:189.
  8. Seitz, S. et al. (2000). Eur J Cancer. 36:1507.
  9. Rennstam, K. et al. (2003). Cancer Res. 63:8861.
  10. Komiya, A. et al. (1997). Jpn J Cancer Res. 88:389.
  11. Lerebours, F. et al. (1999). Int J Cancer. 81:854.
  12. Kahng, Y.S. et al. (2003) J Gastroenterol Hepatol. 18:430.
  13. Hou, S. et al. (2013). Hum Mutat. 34:74.
  14. Fujikado N. et al. (2006). Arthritis Res.& Ther. 8:R100.
  15. Kawata, K. et al. (2017) J Cell Biochemistry. 118:4033.
Long Name
Platelet-derived Growth Factor Receptor-like
Entrez Gene IDs
5157 (Human); 68797 (Mouse); 290771 (Rat)
Alternate Names
PDGFRL; PDGFR-Like Protein; PDGRL; platelet-derived growth factor receptor-like protein; platelet-derived growth factor receptor-like; platelet-derived growth factor-beta-like tumor suppressor; PRLTS; PRLTSPDGF receptor beta-like tumor suppressor

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