Mouse PDGFRL Antibody (1010639)

R&D Systems | Catalog # MAB9739

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 1010639
View all PDGFRL Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived mouse PDGFRL
Gln22-Ser375
Accession # Q6PE55

Specificity

Detects mouse PDGFRL in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse PDGFRL Antibody (1010639)

Cell Proliferation Induced by PDGFRL and Neutralization by Mouse PDGFRL Antibody.

Cell Proliferation Induced by PDGFRL and Neutralization by Mouse PDGFRL Antibody.

Recombinant Mouse PDGFRL (Catalog # 9739-PR) stimulates proliferation in the HCT-116 human colorectal carcinoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse PDGFRL (10 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse PDGFRL Monoclonal Antibody (Catalog # MAB9739). The ND50 is typically 4-24 µg/mL.

Applications for Mouse PDGFRL Antibody (1010639)

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize PDGFRL-induced proliferation in the HCT-116 human colorectal carcinoma cell line. The Neutralization Dose (ND50) is typically 4-24 µg/mL in the presence of 10 μg/mL Recombinant Mouse PDGFRL.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGFRL

Platelet-derived growth factor receptor-like protein (PDGFRL) is a 67 kDa glycoprotein protein consisting of two Ig-like C2-type domains. By sequence similarity, mouse PDGFRL is 90% similar to the human version and 95% similar to that of the rat. It is a secreted protein related to the class III subfamily of receptor tyrosine kinases (RTK), the platelet-derived growth factor receptors (1-5). The high frequency of loss of heterozygosity (LOH) near the gene of PDGFRL indicated in several studies that PDGFRL can be a tumor suppressor gene (TSG) in breast cancer (6-9), colorectal cancer, prostate cancer (10), non-small cell lung cancers (11) and hepatocellular carcinoma (12). A variant of PDGFRL is found to play a role in the development of Behçet disease, a complex immunoregulatory disease (13). The autoimmune role of PDGFRL is also supported by its up-regulation in a mouse model for Rheumatoid Arthritis (14). Another study also showed that PDGFRL may play a role in chondrocyte proliferation and differentiation (15).

References

  1. Guo, F-J. et al. (2010). World J Gastroenterol. 16:1465.
  2. Andrae, J. et al. (2008) Genes Dev. 22:1276.
  3. Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
  4. Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
  5. Matsui, T. et al. (1989) Science 243:800.
  6. Seitz, S. et al. (2006). Genes Chromosomes Cancer 45:612.
  7. Yaremko, M.L. et al. (1996). Genes, Chromosome Cancer 16:189.
  8. Seitz, S. et al. (2000). Eur J Cancer. 36:1507.
  9. Rennstam, K. et al. (2003). Cancer Res. 63:8861.
  10. Komiya, A. et al. (1997). Jpn J Cancer Res. 88:389.
  11. Lerebours, F. et al. (1999). Int J Cancer. 81:854.
  12. Kahng, Y.S. et al. (2003) J Gastroenterol Hepatol. 18:430.
  13. Hou, S. et al. (2013). Hum Mutat. 34:74.
  14. Fujikado N. et al. (2006). Arthritis Res.& Ther. 8:R100.
  15. Kawata, K. et al. (2017) J Cell Biochemistry. 118:4033.

Long Name

Platelet-derived Growth Factor Receptor-like

Alternate Names

PDGFR-Like Protein, PDGRL, PRLTS

Entrez Gene IDs

5157 (Human); 68797 (Mouse); 290771 (Rat)

Gene Symbol

PDGFRL

UniProt

Q6PE55

Additional PDGFRL Products

Product Documents for Mouse PDGFRL Antibody (1010639)

Certificate of Analysis

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Product Specific Notices for Mouse PDGFRL Antibody (1010639)

For research use only

Citations for Mouse PDGFRL Antibody (1010639)

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