|Detection of Mouse PEAR1 by Western Blot. Western blot shows lysates of mouse spleen tissue and mouse platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse PEAR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7607) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PEAR1 at approximately 120-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Detection of PEAR1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Sheep Anti-Mouse PEAR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7607, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).|
PEAR1 (Platelet Endothelial Aggregation Receptor 1; Also Jedi/Jagged and Delta protein and MEGF12) is a 140-160 kDa member of the MEGF/Multiple Epidermal Growth Factor domain family of molecules.It has restricted expression, being found on megakaryocytes/platelets, vascular endothelial cells, early hematopoietic stem cells and dorsal root ganglia satellite glial cells. PEAR1 has an extracellular domain (ECD) similar to Jagged1, and soluble PEAR1 is known to be an inhibitor of notch signaling. Thus, it likely participates in blood cell differentiation. It also is reported to bind to apoptotic neurons, facilitating clearance during development. Mature mouse PEAR1 is a 1016 amino acid (aa) type I transmembrane protein. It possesses a 736 aa ECD (aa 19-754) plus a 259 aa cytoplasmic region (aa 776-1034). The ECD contains one EMI (Emilin) domain (aa 25-137), followed by 9 EGF-like repeats (aa 181-691). There are at least three utilized phosphorylation sites in the cytoplasmic tail. Three isoform variants exist. One shows a deletion of aa 375-404, a second contains an 11 aa substitution for aa 737-747, and a third utilizes an alternative start site at Met137. Over aa 19-754, mouse PEAR1 shares 94% and 85% aa sequence identity with rat and human PEAR1, respectively.