Mouse Persephin Antibody

Catalog # Availability Size / Price Qty
MAB2479
MAB2479-SP

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Mouse Persephin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Persephin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) AgRP, rmArtemin, rmCripto, recombinant drosophila DPP, recombinant human DPP-6, rhGDNF, rhLAP, rmMIS, rmNodal, rhTGF‑ alpha, rhTGF-beta 1, rhTGF-beta 1.2, rhTGF-beta 2, rhTGF-beta 3, or recombinant amphibian TGF-beta 5 is observed.
Source
Monoclonal Rat IgG2A Clone # 240839
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Persephin
Ala61-Gly156
Accession # O70300
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Persephin (Catalog # 2479-PS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Persephin

Persephin is a secreted protein belonging to the glial cell line‑derived neurotrophic factor (GDNF) family of the TGF-beta superfamily. It shares 38‑46% amino acid identity with family members GDNF, neurturin and artemin. Persephin is expressed at very low levels in most tissues (1). The 10‑12 kDa mature protein contains several cysteines that are conserved among family members. It circulates as an unglycosylated disulfide-linked homodimer. Mature mouse persephin shares 94%, 81%, 79% and 76% amino acid sequence identity with rat, human, bovine and canine persephin, respectively. Like other GDNF family members, persephin acts through engagement of a glycosylphosphatidylinositol (GPI)-linked GDNF receptor family (GRF) member that signals through the receptor tyrosine kinase RET. Persephin is reported to promote both the survival and growth of central dopaminergic and motor neurons, and kidney development (1). These effects are correlated with the expression patterns of its specific receptor, GFR alpha 4, and RET (2, 3). Functional GFR alpha 4 isoforms are found only in thyroid, adrenal medulla and portions of the central nervous system and include GPI-linked, transmembrane and soluble forms (3, 4). In vitro, persephin promotes survival only in neurons which coexpress GPI-linked GFR alpha 4 with RET (2, 5). This effect does not show a strong correlation to the recruitment of RET in lipid rafts seen with other GDNF family members (6). Disruption of the persephin gene results in mice that are morphologically normal but have more damage and less effective repair after a central nervous system insult simulating a stroke. Microinjection of persephin prior to treatment protects against damage in both wild-type and mutant mouse brains, but surprisingly, high doses of persephin are detrimental (7).

References
  1. Milbrandt, J. et al. (1998) Neuron 20:245.
  2. Lindahl, M. et al. (2001) J. Biol. Chem. 276:9344.
  3. Lindahl, M. et al. (2000) Mol. Cell. Neurosci. 15:522.
  4. Akerud, P. et al. (2002) Mol. Cell. Neurosci. 21:205.
  5. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
  6. Yang, J. et al. (2004) FEBS Lett. 569:267.
  7. Tomac, A. C. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9521.
Entrez Gene IDs
5623 (Human); 19197 (Mouse)
Alternate Names
Persephin; PSP; PSPN

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