PGRP-L (peptidoglycan recognition protein‑long; also called PGRP-2 or TagL) is a 530 amino acid (aa) member of the PGRP family that is expressed in the liver. All mouse PGRPs bind peptidoglycan and Gram-positive bacteria, and may function as innate immunity pattern recognition molecules. PGRP-L is a Zn2+-dependent N‑acetylmuramoyl-L-alanine amidase that hydrolyzes cell wall glycopeptides. It contains two membrane-spanning segments, with both termini thought to be extracellular, but is also reported in serum. A truncated 450 aa isoform has been reported. Overall aa identity between mouse and human PGRP-L is 68%.
Mouse PGLYRP2/PGRP-L Antibody
R&D Systems | Catalog # MAB4704
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 439728
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PGLYRP2/PGRP-L
Ser23-Asn530
Accession # Q8VCS0
Ser23-Asn530
Accession # Q8VCS0
Specificity
Detects mouse PGLYRP2/PGRP-L in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) PGRP-S and rmPGRP-1 beta is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse PGLYRP2/PGRP-L Antibody
Detection of PGLYRP2/PGRP-L in Mouse Liver Cells by Flow Cytometry.
Mouse liver single-cell suspension was stained with Rat Anti-Mouse PGLYRP2/PGRP-L Monoclonal Antibody (Catalog # MAB4704, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Mouse PGLYRP2/PGRP-L Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Mouse liver single‑cell suspension fixed with paraformaldehyde and permeabilized with saponin
Sample: Mouse liver single‑cell suspension fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: Recombinant Mouse PGLYRP2/PGRP-L
Sample: Recombinant Mouse PGLYRP2/PGRP-L
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PGLYRP2/PGRP-L
Long Name
Peptidoglycan Recognition Protein Long/Peptidoglycan Recognition Protein 2
Alternate Names
PGLYRPL, PGRP-L, PGRP2, PGRPL, TAGL
Gene Symbol
PGLYRP2
UniProt
Additional PGLYRP2/PGRP-L Products
Product Documents for Mouse PGLYRP2/PGRP-L Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse PGLYRP2/PGRP-L Antibody
For research use only
Citations for Mouse PGLYRP2/PGRP-L Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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